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Why the difference in sample order affects the sctransform results #150

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yuxuanChen777 opened this issue Oct 24, 2022 · 3 comments
Open

Why the difference in sample order affects the sctransform results #150

yuxuanChen777 opened this issue Oct 24, 2022 · 3 comments

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@yuxuanChen777
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I entered the same cellular gene matrix, just changed the order of the cells before and after, why the sctransform results are different
@yuxuanChen777
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image
The var.features found by the sctransform are different after the order of the samples is distorted

@yuxuanChen777
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Please tell me the reasons for this randomness

@lc-hhh
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lc-hhh commented Apr 5, 2023

Actually, I notice there is a parameter seed.use in SCTransform function. By default, sets the seed to 1448145.

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@yuxuanChen777 @lc-hhh