No feature overlap between existing object and new layer data

[mainyanghr]

Please see the code, It seems my object does not have overlap with reference. How should I improve?

I am also confused with Normalization step, In the previous Vignette, it was suggested to use STC transormation.

names(object)
[1] "RNA" "HTO" "ADT" "RNA_nn" "RNA_snn" "wknn" "wsnn" "pca"
[9] "umap" "harmony" "apca" "wnn.umap" "rna.umap" "adt.umap"

> names(object@assays)
[1] "RNA" "HTO" "ADT"

**> objquery <- RunAzimuth(object,

•         reference = "pbmcref")**
  

Warning: Overwriting miscellanous data for modelWarning: Adding a dimensional reduction (refUMAP) without the associated assay being presentWarning: Adding a dimensional reduction (refUMAP) without the associated assay being presentdetected inputs from HUMAN with id type Gene.stable.ID
reference rownames detected HUMAN with id type Gene.name
Found 0 out of 60 total inputs in conversion table
Error: No feature overlap between existing object and new layer data
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