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How to generate reference with my own single-cell RNA-seq datasets #18

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yingsun-ucsd opened this issue Apr 5, 2023 · 0 comments
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yingsun-ucsd commented Apr 5, 2023

I used Seurat for my image-based spatial data from nanostring. It works great. Instead of performing unsupervised analysis, I followed the instructions to map the profiles to Azimuth Human Pancreas reference as "pancreas.object <- RunAzimuth( pancreas.object, reference = "pancreasref" )" Thanks for @AustinHartman's help.

Now I would like to do the same thing with my own single-cell RNA-seq data as the reference. To my understanding, I can use "AzimuthReference" to generate the reference to do so. But I did not find the instruction, protocol, or example of how. I am very new to Seurat, could you please give me some guidance on it? Thanks a lot!

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