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Really cool tool and web portal! I was trying to understand how you have been building references for Azimuth. A lot of what you did makes sense to me, but I stumbled across a line in the human BMMC reference script indicating an additional normalization step after individual batches had already been scTransformed.
Hi!
Really cool tool and web portal! I was trying to understand how you have been building references for Azimuth. A lot of what you did makes sense to me, but I stumbled across a line in the human BMMC reference script indicating an additional normalization step after individual batches had already been scTransformed.
See:
azimuth-references/human_bonemarrow/scripts/integrate.R
Line 58 in 536fcbb
I was wondering why the second normalization step is needed here?
Thanks a lot!
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