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USVSEG: GUI-based MATLAB script for robust segmentation of rodents' ultrasonic vocalizations

Installation

Just download the latast version of matlab file (.m), and run from matlab.

Python version of USVSEG is also available here.

User's guide

Made by Dr. Kouta Kanno (https://cannonolab.com/en/)

Output description

Segmented WAV files

Image (JPG or TIF) files of segmented spectrogram

CSV file

contains onset and offset time points and an additional three acoustical features for each segmented syllable:

  • duration
  • maxfreq (max frequency) ... peak frequency of the time frame that has the highest amplitude in that syllable
  • maxamp (max amplitude) ... amplitude value of the maxfreq
  • meanfreq (mean frequency) ... mean of peak frequencies
  • cvfreq (cv of frequency) ... coefficient of variaction of peak frequencies

Peak frequency trace

Other info

Brief presentation for USVSEG (in Japanese) in SlideShare https://www.slideshare.net/ryosuketachibana12/usvseg
A part of mice USV in the dataset was obtained from Data Sharing Platform of "Individuality" project. http://www.google.com/url?q=http%3A%2F%2Fdata-share.koseisouhatsu.jp%2Fdata%2Fsearch%2Findex.action&sa=D&sntz=1&usg=AFQjCNHZLc0KZQVIKIkzWIW0EDj44OFlKw

License

This software is released under the MIT License, see LICENSE.txt.

Citation

Original idea and processing procedures of USVSEG have been published in an open-access journal.
Please cite this paper when you publish research works with useing this software:
Tachibana RO, Kanno K, Okabe S, Kobayasi KI, Okanoya K (2020) USVSEG: A robust method for segmentation of ultrasonic vocalizations in rodents. PloS one 15(2) e0228907.