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ome.tiff saving + swap dimension #24

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sdaste opened this issue Feb 16, 2024 · 1 comment
Open

ome.tiff saving + swap dimension #24

sdaste opened this issue Feb 16, 2024 · 1 comment
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enhancement New feature or request

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@sdaste
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sdaste commented Feb 16, 2024

Hi,

First thank you for this great package!

Flow_registration outputs multi-channel TIFF stack with dimensions frame x (spatial_1) x (spatial_2) x channel. However, Suite2P (package for cell segmentation) expects frame x channel x (spatial_1) x (spatial_2) dimensions in TIFF stack with 2 channels.
Tiff larger than 4Go cannot be read by it either, and ome.tiff files solve this problem.

Would it therefore be possible to swap dimensions as above and save the result in ome.tiff?

Thanks!

@phflot
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phflot commented Feb 16, 2024

Thank you for the feedback! I was not aware about ome.tiff, I'll try to look into this over the weekend and will add an option for suite2p compatible tiff files. For the meantime, there is also the "MULTIFILE_TIFF" option which outputs one file per channel, in case that helps.
Best wishes,
Philipp

@phflot phflot self-assigned this Feb 16, 2024
@phflot phflot added the enhancement New feature or request label Feb 16, 2024
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