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cellranger mkref with multiple species #319

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MaestSi opened this issue Apr 16, 2024 · 0 comments
Open

cellranger mkref with multiple species #319

MaestSi opened this issue Apr 16, 2024 · 0 comments
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enhancement New feature or request

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@MaestSi
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MaestSi commented Apr 16, 2024

Description of feature

Dear developers,
in cellranger manual, it is reported that parameters --genome, --fasta and --genes of cellranger mkref can be specified multiple times, for each of the genomes that need to be merged. However, this feature does not seem to be available in scrnaseq v2.5.1 pipeline. It looks like users can create their own reference by running cellranger mkref outside of the scrnaseq pipeline, and then run it using --cellranger_index parameter instead of --genome_fasta and --gtf parameters. However, it would be nice to have this feature integrated in the pipeline.
Best,
SM

@MaestSi MaestSi added the enhancement New feature or request label Apr 16, 2024
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