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i am trying to analyse ATAC-seq data with snakepipes. Currently, I am facing an error during the DNA-mapping workflow. Before that, I have created an index for GRCm38. I have crossed samples from CAST_EiJ and C57BL_6NJ. Thus, I use the allelic-mapping mode and pass a SNP file to the pipeline:
rule bowtie2_index:
input: snp_genome/CAST_EiJ_C57BL_6NJ_dual_hybrid.based_on_GRCm38_105_Mapping_N-masked
output: snp_genome/bowtie2_Nmasked/Genome.1.bt2
log: snp_genome/bowtie2_Nmasked/bowtie2.index.out, snp_genome/bowtie2_Nmasked/bowtie2.index.err
jobid: 7
threads: 5
resources: tmpdir=/projects/allelespecificchromatinmouse/work/MouseSeqData/temp
Activating conda environment: /home/jrummel/anaconda3/envs/97bf6a3bf6520594fcbd63a07735fa20
[Tue Oct 18 17:05:16 2022]
Error in rule bowtie2_index:
jobid: 7
output: snp_genome/bowtie2_Nmasked/Genome.1.bt2
log: snp_genome/bowtie2_Nmasked/bowtie2.index.out, snp_genome/bowtie2_Nmasked/bowtie2.index.err (check log file(s) for error message)
conda-env: /home/jrummel/anaconda3/envs/97bf6a3bf6520594fcbd63a07735fa20
shell:
bowtie2-build --threads 5 snp_genome/bowtie2_Nmasked/Genome > snp_genome/bowtie2_Nmasked/bowtie2.index.out 2> snp_genome/bowtie2_Nmasked/bowtie2.index.err
(one of the commands exited with non-zero exit code; note that snakemake uses bash strict mode!)
Job failed, going on with independent jobs.
Exiting because a job execution failed. Look above for error message
Complete log: /projects/allelespecificchromatinmouse/work/MouseSeqData/results/DNA-Mapping/F2022YB/.snakemake/log/2022-10-18T160358.600796.snakemake.log
!!! ERROR in DNA mapping workflow! !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
bowtie2.index.err:
No output file specified!
Bowtie 2 version 2.3.5.1 by Ben Langmead (langmea@cs.jhu.edu, www.cs.jhu.edu/~langmea)
Usage: bowtie2-build [options]* <reference_in> <bt2_index_base>
reference_in comma-separated list of files with ref sequences
bt2_index_base write bt2 data to files with this dir/basename
*** Bowtie 2 indexes work only with v2 (not v1). Likewise for v1 indexes. ***
Options:
-f reference files are Fasta (default)
-c reference sequences given on cmd line (as
<reference_in>)
--large-index force generated index to be 'large', even if ref
has fewer than 4 billion nucleotides
--debug use the debug binary; slower, assertions enabled
--sanitized use sanitized binary; slower, uses ASan and/or UBSan
--verbose log the issued command
-a/--noauto disable automatic -p/--bmax/--dcv memory-fitting
-p/--packed use packed strings internally; slower, less memory
--bmax <int> max bucket sz for blockwise suffix-array builder
--bmaxdivn <int> max bucket sz as divisor of ref len (default: 4)
--dcv <int> diff-cover period for blockwise (default: 1024)
--nodc disable diff-cover (algorithm becomes quadratic)
-r/--noref don't build .3/.4 index files
-3/--justref just build .3/.4 index files
-o/--offrate <int> SA is sampled every 2^<int> BWT chars (default: 5)
-t/--ftabchars <int> # of chars consumed in initial lookup (default: 10)
--threads <int> # of threads
--seed <int> seed for random number generator
-q/--quiet verbose output (for debugging)
-h/--help print detailed description of tool and its options
--usage print this usage message
--version print version information and quit
bowtie2.index.out is empty.
Do you have any idea how to fix this? If you need more information just let me know.
Thanks a lot for your help :)
(I have seen issue #517 regarding the same problem. Unfortunately that did not help.)
Best
Julian
The text was updated successfully, but these errors were encountered:
thanks for reporting this issue.
It looks like the N-masked fasta file required for the bowtie index is missing. It should have been generated in the previous step. Did rule create_snpgenome produce any errors?
Both files, SNPsplit_createSNPgenome.out & SNPsplit_createSNPgenome.err, are empty.
The directory snp_genome/CAST_EiJ_C57BL_6NJ_dual_hybrid.based_on_GRCm38_105_Mapping_N-masked contains N-masked fasta files for all chromosomes.
Hi everyone,
i am trying to analyse ATAC-seq data with snakepipes. Currently, I am facing an error during the DNA-mapping workflow. Before that, I have created an index for GRCm38. I have crossed samples from CAST_EiJ and C57BL_6NJ. Thus, I use the allelic-mapping mode and pass a SNP file to the pipeline:
DNA-mapping -i /projects/allelespecificchromatinmouse/work/ATAC/X204SC21092819-Z01-F001_01/raw_data/F2022YB/ -o /projects/allelespecificchromatinmouse/work/MouseSeqData/results/DNA-Mapping/F2022YB/ --local --mode allelic-mapping --VCFfile /projects/allelespecificchromatinmouse/work/MouseSeqData/help_data/mgp.v5.merged.snps_all.dbSNP142.vcf --strains 'CAST_EiJ,C57BL_6NJ' --ext .fq.gz --reads '_1' '_2' GRCm38_105_Mapping
I get the following Error-message:
bowtie2.index.err:
bowtie2.index.out is empty.
Do you have any idea how to fix this? If you need more information just let me know.
Thanks a lot for your help :)
(I have seen issue #517 regarding the same problem. Unfortunately that did not help.)
Best
Julian
The text was updated successfully, but these errors were encountered: