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start codon position problem #31

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linzhi2013 opened this issue Nov 9, 2019 · 0 comments
Open

start codon position problem #31

linzhi2013 opened this issue Nov 9, 2019 · 0 comments

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@linzhi2013
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这个应该跟参考数据库里面的蛋白基因本身的注释存在问题有关。应该是由于数据库里面的这个基因,由于他们上传的注释结果就是在目前mitoz给出的注释结果的后面(如果该基因在正链的话),导致mitoz将参考蛋白与老师您的序列比对时候,也只能比对到这个位置。

mitoz 会自动往前找起始密码子,找到第一个之后,就停止了。

老师的想法是,只要能够往前延伸,就把坐标不断往前推?即使最终预测到的基因长度很长?

因为参考数据库里边的长度也未必对

如果参考数据库本身有问题呢

只是我认为细菌 线粒体什么的 不会平白无故多出来一些非编码区域

你也考虑一下吧 其实这东西最好有转录组能验证
但我的延长规律也是我一己之见。

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