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Update Variant Calling workflow #212

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Talishask opened this issue May 27, 2020 · 0 comments
Open

Update Variant Calling workflow #212

Talishask opened this issue May 27, 2020 · 0 comments

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@Talishask
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I'm a member of The Carpentries staff and I'm submitting this issue on behalf of another member of the community. In most cases I won't be able to follow up or provide more details other that what I'm providing below.


In the Data Carpentry Genomics Workshop in the Data Wrangling and Processing lesson, the fourth section is Variant Calling workflow. Under Step 2: Detect the single nucleotide polymorphisms (SNPs), the output file in the command:

$ bcftools call --ploidy 1 -m -v -o results/bcf/SRR2584866_variants.vcf results/bcf/SRR2584866_raw.bcf

The output file is a .vcf file and is put in the results/bcf/ directory. I am just wondering whether this file should be put in the results/vcf directory instead. This ensures students correctly follow organisation of files.

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