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FAIMS-DDA protein assembling error #50

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fedefratini opened this issue Oct 24, 2023 · 1 comment
Open

FAIMS-DDA protein assembling error #50

fedefratini opened this issue Oct 24, 2023 · 1 comment

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@fedefratini
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Describe the bug
I'm using for the first time FAIMS data, with 2 or 3 CV. I divided in 2-3 raw files so there is one CV for raw file. The run stop during the protein assembling step and MQ closes. I just downloaded the last version, but it gives exactly the same error, that I can't understand how to solve. it seems a file missing. All reposrts are attached
Assembling_proteins 0116.comment.txt
Assembling_proteins 0116.error.txt
#runningTimes.txt
mqpar_FAIMS-DDA.txt

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  • Version [e.g. 22]

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@fedefratini
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Hi tried also converting raw file in mzxml but it gives
exactly the same error, could you please suggest how to overcome it?

thanks
Federica

id 0
start 06/11/2023 15:44:17
title Assembling_proteins (01/16)
description D:\test-faims\mzxml\combined\proc Assembling_proteins 0 Assembling_proteins (01/16) Process 46 0 D:\test-faims\mzxml\combined D:\test-faims\mzxml\mqpar.xml 0
error D:\test-faims\mzxml\combined\proc Assembling_proteins 0 Assembling_proteins (01/16) Process 46 0 D:\test-faims\mzxml\combined D:\test-faims\mzxml\mqpar.xml 0_Could not find file 'D:\test-faims\mzxml\combined\matchesFilePos'._ at System.IO.FileStream.ValidateFileHandle(SafeFileHandle fileHandle)__ at System.IO.FileStream.CreateFileOpenHandle(FileMode mode, FileShare share, FileOptions options)__ at System.IO.FileStream..ctor(String path, FileMode mode, FileAccess access, FileShare share, Int32 bufferSize, FileOptions options)__ at System.IO.FileStream..ctor(String path, FileMode mode, FileAccess access, FileShare share)__ at BaseLibS.Util.FileUtils.GetBinaryReader(String path)__ at MaxQuantLibS.Domains.Peptides.ProteinTasks.ProteinAssembly.ProcessPeptides(String combinedFolder, MaxQuantParams mqpar, Int32 fileIndex, IDictionary`2 andromedaScorePositioningCache, Responder responder)__ at MaxQuantLibS.Domains.Peptides.ProteinTasks.ProteinAssembly.ProcessPeptidesX(String combinedFolder, String mqparFile, Int32 jobIndex, Responder responder)__ at MaxQuantLibS.Domains.Peptides.Work.ProteinAssembler.Calculation(String[] args, Responder responder)__ at MaxQuantLibS.Domains.Peptides.Work.MaxQuantWorkDispatcherUtil.PerformTask(Int32 taskType, String[] args, Responder responder)__ at MaxQuantLibS.Base.MaxQuantUtils.Run(Int32 softwareId, Int32 taskType, String[] args, Responder responder)__ at MaxQuantTaskCore.Program.Function(String[] args, Responder responder)__ at Utils.Util.ExternalProcess.Run(String[] args, Boolean debug)
end 06/11/2023 15:44:24

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