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Fermentation substrates are not being picked up as fermentation substrates and are being reported under tests. It's OK if they are outputted into both categories, but if a sentence begins with "All strains ferment LIST OF COMPOUNDS" then the whole list of compounds should all be outputted as Fermentation Substrates Used.
Also, not all the fermentation substrates are being correctly picked up (see below for what the output should look like). For example, 2-ketogluconate, 5-ketogluconate, β-gentiobiose are not being picked up at all.
For the sentences: "Gram-positive, motile, non-sporeforming rods of 0.89–1.20 µm width by 1.18–3.48 µm length. Cells are found singly, in pairs and in short chains. It is aerotolerant. Colonies on MRS agar after 4 days incubation at 28 ˚C are 1–1.25 mm in diameter, white, smooth, circular and with entire edges. It is catalase negative. Growth occurs at pH 4.5 and 8 and in the presence of 5% (w/v) NaCl, but neither at pH3.3, nor with 10% (w/v) NaCl. Obligate homofermentative: gluconate and pentoses are not fermented and glucose is fermented but no gas is released. L-lactate is produced as exclusive end product from hexoses. Ammonia is not produced from arginine, and mannitol is not formed from fructose. Exopolysaccharide is produced from sucrose. All strains ferment glucose, fructose, mannose, mannitol, methyl α-D-glucoside, N-acetyl-glucosamine, amygdalin, arbutin, salicin, maltose, sucrose, trehalose, β-gentiobiose and D-turanose, and hydrolyze aesculin. The isolates do not ferment glycerol, erythritol, D-arabinose, L-arabinose, ribose, D-xylose, L-xylose, adonitol, methyl β-xyloside, galactose, rhamnose, dulcitol, inositol, methyl α-D-mannoside, lactose, melibiose, inulin, melezitose, D-raffinose, starch, glycogen, xylitol, D-lyxose, D-tagatose, D-fucose, L-fucose, D-arabitol, L-arabitol, gluconate, 2-ketogluconate or 5-ketogluconate.
Fermentation substrates are not being picked up as fermentation substrates and are being reported under tests. It's OK if they are outputted into both categories, but if a sentence begins with "All strains ferment LIST OF COMPOUNDS" then the whole list of compounds should all be outputted as Fermentation Substrates Used.
Also, not all the fermentation substrates are being correctly picked up (see below for what the output should look like). For example, 2-ketogluconate, 5-ketogluconate, β-gentiobiose are not being picked up at all.
For the sentences: "Gram-positive, motile, non-sporeforming rods of 0.89–1.20 µm width by 1.18–3.48 µm length. Cells are found singly, in pairs and in short chains. It is aerotolerant. Colonies on MRS agar after 4 days incubation at 28 ˚C are 1–1.25 mm in diameter, white, smooth, circular and with entire edges. It is catalase negative. Growth occurs at pH 4.5 and 8 and in the presence of 5% (w/v) NaCl, but neither at pH3.3, nor with 10% (w/v) NaCl. Obligate homofermentative: gluconate and pentoses are not fermented and glucose is fermented but no gas is released. L-lactate is produced as exclusive end product from hexoses. Ammonia is not produced from arginine, and mannitol is not formed from fructose. Exopolysaccharide is produced from sucrose. All strains ferment glucose, fructose, mannose, mannitol, methyl α-D-glucoside, N-acetyl-glucosamine, amygdalin, arbutin, salicin, maltose, sucrose, trehalose, β-gentiobiose and D-turanose, and hydrolyze aesculin. The isolates do not ferment glycerol, erythritol, D-arabinose, L-arabinose, ribose, D-xylose, L-xylose, adonitol, methyl β-xyloside, galactose, rhamnose, dulcitol, inositol, methyl α-D-mannoside, lactose, melibiose, inulin, melezitose, D-raffinose, starch, glycogen, xylitol, D-lyxose, D-tagatose, D-fucose, L-fucose, D-arabitol, L-arabitol, gluconate, 2-ketogluconate or 5-ketogluconate.
MicroPIE extracted for fermentation substrates used:
glucose | exclusive end product | hexoses | fructose | mannitol | sucrose
fermentation substrates should be:
glucose, fructose, mannose, mannitol, methyl α-D-glucoside, N-acetyl-glucosamine, amygdalin, arbutin, salicin, maltose, sucrose, trehalose, β-gentiobiose, D-turanose
MicroPIE extracted for fermentation substrates not used:
fermented | gluconate | pentoses | arginine | D-arabinose | D-arabitol | D-fucose | D-lyxose | D-raffinose | D-tagatose | D-xylose | L-arabinose | L-arabitol | L-fucose | L-xylose | adonitol | dulcitol | erythritol | galactose | glycerol | glycogen | inositol | inulin | lactose | melezitose | melibiose | methyl α-D-mannoside | methyl β-xyloside | rhamnose | ribose | starch | xylitol
fermentation substrates not used should be:
gluconate, pentoses, glycerol, erythritol, D-arabinose, L-arabinose, ribose, D-xylose, L-xylose, adonitol, methyl β-xyloside, galactose, rhamnose, dulcitol, inositol, methyl α-D-mannoside, lactose, melibiose, inulin, melezitose, D-raffinose, starch, glycogen, xylitol, D-lyxose, D-tagatose, D-fucose, L-fucose, D-arabitol, L-arabitol, gluconate, 2-ketogluconate, 5-ketogluconate
MicroPIE extracts for tests positive:
sucrose | aesculin | D-turanose | trehalose | maltose | salicin | arbutin | amygdalin | N-acetyl-glucosamine | mannitol | mannose | fructose | strains ferment glucose
tests positive should be:
aesculin
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