Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

manta_run_workflow fails with Unexpected character in reference sequence. #31

Open
Parsoa opened this issue May 17, 2020 · 0 comments
Open

Comments

@Parsoa
Copy link

Parsoa commented May 17, 2020

I get the following error during the manta_run_workflow stage:

[2020-05-17T19:56:16.703129Z] [23217_1] [WorkflowRunner] [ERROR] [makeLocusGraph_chromId_438_chr19_KI270933v1_alt_0000] Error Message:
[2020-05-17T19:56:16.705237Z] [23217_1] [WorkflowRunner] [ERROR] [makeLocusGraph_chromId_438_chr19_KI270933v1_alt_0000] Last 6 stderr lines from task (of 6 total lines):
[2020-05-17T19:56:16.705237Z] [23217_1] [WorkflowRunner] [ERROR] [2020-05-17T19:53:07.332513Z] [23217_1] [makeLocusGraph_chromId_438_chr19_KI270933v1_alt_0000] ERROR::
Unexpected character in reference sequence.
[2020-05-17T19:56:16.705237Z] [23217_1] [WorkflowRunner] [ERROR] [2020-05-17T19:53:07.334080Z] [23217_1] [makeLocusGraph_chromId_438_chr19_KI270933v1_alt_0000]   refer$
nce_sequence_file: '/share/data/BayesTyper/bayestyper_GRCh38_bundle_v1.3/GRCh38.fa'
[2020-05-17T19:56:16.705237Z] [23217_1] [WorkflowRunner] [ERROR] [2020-05-17T19:53:07.346773Z] [23217_1] [makeLocusGraph_chromId_438_chr19_KI270933v1_alt_0000]   chrom$
some: 'chr19_KI270933v1_alt'
[2020-05-17T19:56:16.705237Z] [23217_1] [WorkflowRunner] [ERROR] [2020-05-17T19:53:07.347916Z] [23217_1] [makeLocusGraph_chromId_438_chr19_KI270933v1_alt_0000]   chara$
ter: '>'
[2020-05-17T19:56:16.705237Z] [23217_1] [WorkflowRunner] [ERROR] [2020-05-17T19:53:07.348516Z] [23217_1] [makeLocusGraph_chromId_438_chr19_KI270933v1_alt_0000]   chara$
ter_decimal_index: 62
[2020-05-17T19:56:16.705237Z] [23217_1] [WorkflowRunner] [ERROR] [2020-05-17T19:53:07.349091Z] [23217_1] [makeLocusGraph_chromId_438_chr19_KI270933v1_alt_0000]   chara$
ter_position_in_chromosome: 118438
gzip: manta/NA19240/results/variants

I get a similar error for all makeLocusGraph workflows launched for alt contigs. The stage eventually fails with the following error:

gzip: manta/NA19240/results/variants/candidateSV.vcf.gz: No such file or directory
gzip: manta/NA19240/results/variants/candidateSV.vcf: No such file or directory

As for this particular case, I checked the sequence for chr19_KI270933v1_alt in the reference file provided by the BayesTyper bundle just in case and it doesn't include any > characters in the reported position. I'm assuming this stage does not look at the BAM file yet, so it shouldn't be a problem with the mapping. Any insights on this? Thanks.

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

1 participant