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Question or Expected behavior
I have a reference genome obtained after CANU assembly, purge_dups and long reads polishing with arrow. I'd like to polish with short reads using Next_polish.
Should I use raw Illumina reads or do some pre-processing (e.g. reads trimming, remove low quality reads ...) of the short reads before using them for polishing ?
Thank you
The text was updated successfully, but these errors were encountered:
Ok good.
My next question is there a recommend depth of coverage for polishing ?
More precisely a maximum depth of coverage because I have close to 500X of Illumina raw reads, but I guess there is no need to use all of it ?
Question or Expected behavior
I have a reference genome obtained after CANU assembly, purge_dups and long reads polishing with arrow. I'd like to polish with short reads using Next_polish.
Should I use raw Illumina reads or do some pre-processing (e.g. reads trimming, remove low quality reads ...) of the short reads before using them for polishing ?
Thank you
The text was updated successfully, but these errors were encountered: