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Module request: Parse Biosciences (Split pipeline) #2145

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mbatiuk opened this issue Oct 20, 2023 · 5 comments · May be fixed by #2180
Open
1 task done

Module request: Parse Biosciences (Split pipeline) #2145

mbatiuk opened this issue Oct 20, 2023 · 5 comments · May be fixed by #2180

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@mbatiuk
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mbatiuk commented Oct 20, 2023

Name of the tool

Parse Biosciences Split pipeline

Tool homepage

https://www.parsebiosciences.com/

Tool description

similar to cellranger, parse biosciences pipeline allowes analysis of parse bioseciences single cell rna sequencing data

Tool output

Current Parse biosciences analysis pipeline can be taken from their support site, account is required:

https://support.parsebiosciences.com/hc/en-us/articles/17166220335636

Summaries of output qc files are in attached files

all_summaries.zip

Log filename pattern

No response

Data suitable for MultiQC plot(s)

Similar to 10x genomics cellranger output

image
image

Most interesting data for the General Stats table

No response

Before submitting

  • I have included example data (zipped, not pasted) that can be used to write the module.
@vladsavelyev
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Thank you!

I started a module here #2180 - so far only parsing the CSV table.

Just wondering if the pipeline outputs and raw data for the plots? So far, the plot data is only available through parsing the hardcoded java script in the HTMLs, which is not ideal, and the HTMLs is pretty huge (>4 MB per sample).

@vladsavelyev vladsavelyev changed the title Include module for parse biosciences split-pipeline Module request: Parse Biosciences (Split pipeline) Nov 15, 2023
@mbatiuk
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mbatiuk commented Dec 7, 2023

sorry for late response, and thank you for developing parse module

here is the full output directory from 200 cell parse run. maybe it will help with getting additional stats and plots:

https://drive.google.com/file/d/1xc2eeIUshezSXLTafGU9xRvZq_SMxt5G/view?usp=drive_link

@vladsavelyev
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Thanks @mbatiuk! I can't seem to open that archive though 🤔

Screenshot 2023-12-13 at 15 42 16

@vladsavelyev vladsavelyev linked a pull request Dec 13, 2023 that will close this issue
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@mbatiuk
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mbatiuk commented Jan 8, 2024

OK, I re-uploaded it, here is the link:

https://drive.switch.ch/index.php/s/Ug3MNDYJfv2xm5H

the issue with access permissions should be solved. if still present - try to unzip, and do chmod -R 777 /UNZIPPED_ARCHIVE_DIRECTORY_ADDRESS

@ewels
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ewels commented Jan 9, 2024

Thanks! The archive is pretty massive (434MB). I just downloaded it and stripped out all of the big raw data files (BAM files, fastq, gene matrices etc). It's now small enough to attach to a GitHub comment (13MB): 200nucs_small.zip

I think that this archive should still contain all files relevant to MultiQC.

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3 participants