Is basecalling during minknow run mandatory? #348
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Hi devs. My setup only has a single gpu with limited vram (6GB). Is it possible to run the sequencing without basecalling and let only readfish use basecaller to pick reads? Can I do post-basecalling on POD5 files generated at the end of the run once adaptive_sampling is finished? Regards. |
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Hi @gokalpcelik - that is absolutely possible yes. If you don't turn on FASTQ/bam output when setting up your run in MinKNOW, only the I hope that helps, let me know if not. |
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Hi @gokalpcelik - that is absolutely possible yes.
If you don't turn on FASTQ/bam output when setting up your run in MinKNOW, only the
pod5
signal files are written out.You can then basecall these after the run.
I hope that helps, let me know if not.
Rory