SysgroOverview.ipynb

{
 "cells": [
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "# idr0001: Sysgro\n",
    "<a href=\"https://idr.openmicroscopy.org/webclient/?show=screen-3\">Sysgro screen</a>\n",
    "<table><tr>\n",
    "<td>A quick look at one of the studies in the IDR, Sysgro.</td>\n",
    "<td><img src=\"https://idr.openmicroscopy.org/webclient/render_image/1230601/6/0/?c=1|50:300$00FF00,2|100:800$0000FF&m=c&p=normal&ia=0&q=0.9\" width=\"200px\"/></td>\n",
    "<td><img src=\"https://idr.openmicroscopy.org/webclient/render_image/1232528/6/0/?c=1|50:300$00FF00,2|100:800$0000FF&m=c&p=normal&ia=0&q=0.9\" width=\"200px\"/></td>\n",
    "<td><img src=\"https://idr.openmicroscopy.org/webclient/render_image/1275942/6/0/?c=1|50:300$00FF00,2|100:800$0000FF&m=c&p=normal&ia=0&q=0.9\" width=\"200px\"/></td>\n",
    "</tr></table>"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "### Dependencies\n",
    " * [Matplotlib](https://matplotlib.org/)\n",
    " * [NumPy](https://www.numpy.org/)\n",
    " \n",
    "The cell below will install dependencies if you choose to run the notebook in [Google Colab](https://colab.research.google.com/notebooks/intro.ipynb#recent=true). "
   ]
  },
  {
   "cell_type": "code",
   "execution_count": null,
   "metadata": {},
   "outputs": [],
   "source": [
    "%pip install idr-py"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 1,
   "metadata": {
    "collapsed": true
   },
   "outputs": [],
   "source": [
    "import matplotlib\n",
    "import numpy as np\n",
    "import matplotlib.pyplot as plt\n",
    "import omero\n",
    "from idr import connection\n",
    "matplotlib.rcParams['figure.figsize'] = (12.0, 8.0)\n"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 2,
   "metadata": {
    "collapsed": true
   },
   "outputs": [],
   "source": [
    "screen = \"idr0001-graml-sysgro/screenA\""
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "### Connect to IDR"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 12,
   "metadata": {
    "collapsed": true
   },
   "outputs": [],
   "source": [
    "conn = connection('idr.openmicroscopy.org')"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "### Load information about Sysgro"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 3,
   "metadata": {
    "collapsed": true
   },
   "outputs": [],
   "source": [
    "idr0001 = [x for x in conn.listScreens(2) if x.name == screen][0]"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 4,
   "metadata": {},
   "outputs": [
    {
     "data": {
      "text/plain": [
       "u'idr0001-graml-sysgro/screenA'"
      ]
     },
     "execution_count": 4,
     "metadata": {},
     "output_type": "execute_result"
    }
   ],
   "source": [
    "idr0001.name"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 5,
   "metadata": {},
   "outputs": [
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "Publication Title\n",
      "A Genomic Multiprocess Survey of Machineries that Control and Link Cell Shape, Microtubule Organization, and Cell-Cycle Progression\n",
      "\n",
      "Screen Description\n",
      "Primary screen of fission yeast knock out mutants looking for genes controlling cell shape, microtubules, and cell-cycle progression. 262 genes controlling specific aspects of those processes are identifed, validated, and functionally annotated.\n"
     ]
    }
   ],
   "source": [
    "print(idr0001.description)"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 11,
   "metadata": {},
   "outputs": [
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "\n",
      "  PubMed ID: 25373780 http://www.ncbi.nlm.nih.gov/pubmed/25373780\n",
      "  Authors: Graml V, Studera X, Lawson JL, Chessel A, Geymonat M, Bortfeld-Miller M, Walter T, Wagstaff L, Piddini E, Carazo-Salas RE.\n",
      "  Imaging Method: spinning disk confocal microscopy\n",
      "\n"
     ]
    }
   ],
   "source": [
    "for ann in idr0001.listAnnotations():\n",
    "    if ann.OMERO_TYPE == omero.model.MapAnnotationI:\n",
    "        annotations = dict(ann.getValue())\n",
    "print(\"\"\"\n",
    "  PubMed ID: %(PubMed ID)s\n",
    "  Authors: %(Publication Authors)s\n",
    "  Imaging Method: %(Imaging Method)s\n",
    "\"\"\" % annotations)"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 7,
   "metadata": {},
   "outputs": [
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "Plates: 192\n",
      "Images: 109728\n"
     ]
    }
   ],
   "source": [
    "plates = 0\n",
    "images = 0\n",
    "for plate in idr0001.listChildren():\n",
    "    plates += 1\n",
    "    for well in plate.listChildren():\n",
    "        images += len(list(well.listChildren()))\n",
    "print(\"Plates:\", plates)\n",
    "print(\"Images:\", images)"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 8,
   "metadata": {
    "collapsed": true,
    "scrolled": true
   },
   "outputs": [],
   "source": [
    "total = None\n",
    "for plate in idr0001.listChildren():\n",
    "    grid = plate.getWellGrid()\n",
    "    single = np.zeros((len(grid), len(grid[0])))\n",
    "    if total is None:\n",
    "        total = np.zeros((len(grid), len(grid[0])))\n",
    "    for x, row in enumerate(grid):\n",
    "        for y, col in enumerate(row):\n",
    "            single[x][y] = grid[x][y].getImage().getROICount()\n",
    "    total += single\n",
    "    break  # Continue to tally all ROIs"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "### Disconnect when done loading data"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 13,
   "metadata": {
    "collapsed": true
   },
   "outputs": [],
   "source": [
    "conn.close()"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {},
   "source": [
    "### Display results"
   ]
  },
  {
   "cell_type": "code",
   "execution_count": 14,
   "metadata": {},
   "outputs": [
    {
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       "        alert('Your browser does not have WebSocket support.' +\n",
       "              'Please try Chrome, Safari or Firefox ≥ 6. ' +\n",
       "              'Firefox 4 and 5 are also supported but you ' +\n",
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       "mpl.figure.prototype._canvas_extra_style = function(canvas_div) {\n",
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       "\n",
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       "mpl.figure.prototype._root_extra_style = function(canvas_div) {\n",
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       "\n",
       "mpl.figure.prototype._init_canvas = function() {\n",
       "    var fig = this;\n",
       "\n",
       "    var canvas_div = $('<div/>');\n",
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       "    canvas_div.attr('style', 'position: relative; clear: both; outline: 0');\n",
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       "    function canvas_keyboard_event(event) {\n",
       "        return fig.key_event(event, event['data']);\n",
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       "    this.canvas_div = canvas_div\n",
       "    this._canvas_extra_style(canvas_div)\n",
       "    this.root.append(canvas_div);\n",
       "\n",
       "    var canvas = $('<canvas/>');\n",
       "    canvas.addClass('mpl-canvas');\n",
       "    canvas.attr('style', \"left: 0; top: 0; z-index: 0; outline: 0\")\n",
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       "    this.canvas = canvas[0];\n",
       "    this.context = canvas[0].getContext(\"2d\");\n",
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       "\tthis.context.webkitBackingStorePixelRatio ||\n",
       "\tthis.context.mozBackingStorePixelRatio ||\n",
       "\tthis.context.msBackingStorePixelRatio ||\n",
       "\tthis.context.oBackingStorePixelRatio ||\n",
       "\tthis.context.backingStorePixelRatio || 1;\n",
       "\n",
       "    mpl.ratio = (window.devicePixelRatio || 1) / backingStore;\n",
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       "    var rubberband = $('<canvas/>');\n",
       "    rubberband.attr('style', \"position: absolute; left: 0; top: 0; z-index: 1;\")\n",
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       "        stop: function(event, ui) {\n",
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       "\n",
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       "    rubberband.mouseup('button_release', mouse_event_fn);\n",
       "    // Throttle sequential mouse events to 1 every 20ms.\n",
       "    rubberband.mousemove('motion_notify', mouse_event_fn);\n",
       "\n",
       "    rubberband.mouseenter('figure_enter', mouse_event_fn);\n",
       "    rubberband.mouseleave('figure_leave', mouse_event_fn);\n",
       "\n",
       "    canvas_div.on(\"wheel\", function (event) {\n",
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       "        event['data'] = 'scroll'\n",
       "        if (event.deltaY < 0) {\n",
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       "            event.step = -1;\n",
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       "\n",
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       "\n",
       "    this.rubberband = rubberband;\n",
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       "    this.rubberband_context.strokeStyle = \"#000000\";\n",
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       "        // Keep the size of the canvas, canvas container, and rubber band\n",
       "        // canvas in synch.\n",
       "        canvas_div.css('width', width)\n",
       "        canvas_div.css('height', height)\n",
       "\n",
       "        canvas.attr('width', width * mpl.ratio);\n",
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       "\n",
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       "    // Set the figure to an initial 600x600px, this will subsequently be updated\n",
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       "    this._resize_canvas(600, 600);\n",
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       "    $(this.rubberband_canvas).bind(\"contextmenu\",function(e){\n",
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       "mpl.figure.prototype._init_toolbar = function() {\n",
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       "\n",
       "        if (!name) {\n",
       "            // put a spacer in here.\n",
       "            continue;\n",
       "        }\n",
       "        var button = $('<button/>');\n",
       "        button.addClass('ui-button ui-widget ui-state-default ui-corner-all ' +\n",
       "                        'ui-button-icon-only');\n",
       "        button.attr('role', 'button');\n",
       "        button.attr('aria-disabled', 'false');\n",
       "        button.click(method_name, toolbar_event);\n",
       "        button.mouseover(tooltip, toolbar_mouse_event);\n",
       "\n",
       "        var icon_img = $('<span/>');\n",
       "        icon_img.addClass('ui-button-icon-primary ui-icon');\n",
       "        icon_img.addClass(image);\n",
       "        icon_img.addClass('ui-corner-all');\n",
       "\n",
       "        var tooltip_span = $('<span/>');\n",
       "        tooltip_span.addClass('ui-button-text');\n",
       "        tooltip_span.html(tooltip);\n",
       "\n",
       "        button.append(icon_img);\n",
       "        button.append(tooltip_span);\n",
       "\n",
       "        nav_element.append(button);\n",
       "    }\n",
       "\n",
       "    var fmt_picker_span = $('<span/>');\n",
       "\n",
       "    var fmt_picker = $('<select/>');\n",
       "    fmt_picker.addClass('mpl-toolbar-option ui-widget ui-widget-content');\n",
       "    fmt_picker_span.append(fmt_picker);\n",
       "    nav_element.append(fmt_picker_span);\n",
       "    this.format_dropdown = fmt_picker[0];\n",
       "\n",
       "    for (var ind in mpl.extensions) {\n",
       "        var fmt = mpl.extensions[ind];\n",
       "        var option = $(\n",
       "            '<option/>', {selected: fmt === mpl.default_extension}).html(fmt);\n",
       "        fmt_picker.append(option)\n",
       "    }\n",
       "\n",
       "    // Add hover states to the ui-buttons\n",
       "    $( \".ui-button\" ).hover(\n",
       "        function() { $(this).addClass(\"ui-state-hover\");},\n",
       "        function() { $(this).removeClass(\"ui-state-hover\");}\n",
       "    );\n",
       "\n",
       "    var status_bar = $('<span class=\"mpl-message\"/>');\n",
       "    nav_element.append(status_bar);\n",
       "    this.message = status_bar[0];\n",
       "}\n",
       "\n",
       "mpl.figure.prototype.request_resize = function(x_pixels, y_pixels) {\n",
       "    // Request matplotlib to resize the figure. Matplotlib will then trigger a resize in the client,\n",
       "    // which will in turn request a refresh of the image.\n",
       "    this.send_message('resize', {'width': x_pixels, 'height': y_pixels});\n",
       "}\n",
       "\n",
       "mpl.figure.prototype.send_message = function(type, properties) {\n",
       "    properties['type'] = type;\n",
       "    properties['figure_id'] = this.id;\n",
       "    this.ws.send(JSON.stringify(properties));\n",
       "}\n",
       "\n",
       "mpl.figure.prototype.send_draw_message = function() {\n",
       "    if (!this.waiting) {\n",
       "        this.waiting = true;\n",
       "        this.ws.send(JSON.stringify({type: \"draw\", figure_id: this.id}));\n",
       "    }\n",
       "}\n",
       "\n",
       "\n",
       "mpl.figure.prototype.handle_save = function(fig, msg) {\n",
       "    var format_dropdown = fig.format_dropdown;\n",
       "    var format = format_dropdown.options[format_dropdown.selectedIndex].value;\n",
       "    fig.ondownload(fig, format);\n",
       "}\n",
       "\n",
       "\n",
       "mpl.figure.prototype.handle_resize = function(fig, msg) {\n",
       "    var size = msg['size'];\n",
       "    if (size[0] != fig.canvas.width || size[1] != fig.canvas.height) {\n",
       "        fig._resize_canvas(size[0], size[1]);\n",
       "        fig.send_message(\"refresh\", {});\n",
       "    };\n",
       "}\n",
       "\n",
       "mpl.figure.prototype.handle_rubberband = function(fig, msg) {\n",
       "    var x0 = msg['x0'] / mpl.ratio;\n",
       "    var y0 = (fig.canvas.height - msg['y0']) / mpl.ratio;\n",
       "    var x1 = msg['x1'] / mpl.ratio;\n",
       "    var y1 = (fig.canvas.height - msg['y1']) / mpl.ratio;\n",
       "    x0 = Math.floor(x0) + 0.5;\n",
       "    y0 = Math.floor(y0) + 0.5;\n",
       "    x1 = Math.floor(x1) + 0.5;\n",
       "    y1 = Math.floor(y1) + 0.5;\n",
       "    var min_x = Math.min(x0, x1);\n",
       "    var min_y = Math.min(y0, y1);\n",
       "    var width = Math.abs(x1 - x0);\n",
       "    var height = Math.abs(y1 - y0);\n",
       "\n",
       "    fig.rubberband_context.clearRect(\n",
       "        0, 0, fig.canvas.width, fig.canvas.height);\n",
       "\n",
       "    fig.rubberband_context.strokeRect(min_x, min_y, width, height);\n",
       "}\n",
       "\n",
       "mpl.figure.prototype.handle_figure_label = function(fig, msg) {\n",
       "    // Updates the figure title.\n",
       "    fig.header.textContent = msg['label'];\n",
       "}\n",
       "\n",
       "mpl.figure.prototype.handle_cursor = function(fig, msg) {\n",
       "    var cursor = msg['cursor'];\n",
       "    switch(cursor)\n",
       "    {\n",
       "    case 0:\n",
       "        cursor = 'pointer';\n",
       "        break;\n",
       "    case 1:\n",
       "        cursor = 'default';\n",
       "        break;\n",
       "    case 2:\n",
       "        cursor = 'crosshair';\n",
       "        break;\n",
       "    case 3:\n",
       "        cursor = 'move';\n",
       "        break;\n",
       "    }\n",
       "    fig.rubberband_canvas.style.cursor = cursor;\n",
       "}\n",
       "\n",
       "mpl.figure.prototype.handle_message = function(fig, msg) {\n",
       "    fig.message.textContent = msg['message'];\n",
       "}\n",
       "\n",
       "mpl.figure.prototype.handle_draw = function(fig, msg) {\n",
       "    // Request the server to send over a new figure.\n",
       "    fig.send_draw_message();\n",
       "}\n",
       "\n",
       "mpl.figure.prototype.handle_image_mode = function(fig, msg) {\n",
       "    fig.image_mode = msg['mode'];\n",
       "}\n",
       "\n",
       "mpl.figure.prototype.updated_canvas_event = function() {\n",
       "    // Called whenever the canvas gets updated.\n",
       "    this.send_message(\"ack\", {});\n",
       "}\n",
       "\n",
       "// A function to construct a web socket function for onmessage handling.\n",
       "// Called in the figure constructor.\n",
       "mpl.figure.prototype._make_on_message_function = function(fig) {\n",
       "    return function socket_on_message(evt) {\n",
       "        if (evt.data instanceof Blob) {\n",
       "            /* FIXME: We get \"Resource interpreted as Image but\n",
       "             * transferred with MIME type text/plain:\" errors on\n",
       "             * Chrome.  But how to set the MIME type?  It doesn't seem\n",
       "             * to be part of the websocket stream */\n",
       "            evt.data.type = \"image/png\";\n",
       "\n",
       "            /* Free the memory for the previous frames */\n",
       "            if (fig.imageObj.src) {\n",
       "                (window.URL || window.webkitURL).revokeObjectURL(\n",
       "                    fig.imageObj.src);\n",
       "            }\n",
       "\n",
       "            fig.imageObj.src = (window.URL || window.webkitURL).createObjectURL(\n",
       "                evt.data);\n",
       "            fig.updated_canvas_event();\n",
       "            fig.waiting = false;\n",
       "            return;\n",
       "        }\n",
       "        else if (typeof evt.data === 'string' && evt.data.slice(0, 21) == \"data:image/png;base64\") {\n",
       "            fig.imageObj.src = evt.data;\n",
       "            fig.updated_canvas_event();\n",
       "            fig.waiting = false;\n",
       "            return;\n",
       "        }\n",
       "\n",
       "        var msg = JSON.parse(evt.data);\n",
       "        var msg_type = msg['type'];\n",
       "\n",
       "        // Call the  \"handle_{type}\" callback, which takes\n",
       "        // the figure and JSON message as its only arguments.\n",
       "        try {\n",
       "            var callback = fig[\"handle_\" + msg_type];\n",
       "        } catch (e) {\n",
       "            console.log(\"No handler for the '\" + msg_type + \"' message type: \", msg);\n",
       "            return;\n",
       "        }\n",
       "\n",
       "        if (callback) {\n",
       "            try {\n",
       "                // console.log(\"Handling '\" + msg_type + \"' message: \", msg);\n",
       "                callback(fig, msg);\n",
       "            } catch (e) {\n",
       "                console.log(\"Exception inside the 'handler_\" + msg_type + \"' callback:\", e, e.stack, msg);\n",
       "            }\n",
       "        }\n",
       "    };\n",
       "}\n",
       "\n",
       "// from http://stackoverflow.com/questions/1114465/getting-mouse-location-in-canvas\n",
       "mpl.findpos = function(e) {\n",
       "    //this section is from http://www.quirksmode.org/js/events_properties.html\n",
       "    var targ;\n",
       "    if (!e)\n",
       "        e = window.event;\n",
       "    if (e.target)\n",
       "        targ = e.target;\n",
       "    else if (e.srcElement)\n",
       "        targ = e.srcElement;\n",
       "    if (targ.nodeType == 3) // defeat Safari bug\n",
       "        targ = targ.parentNode;\n",
       "\n",
       "    // jQuery normalizes the pageX and pageY\n",
       "    // pageX,Y are the mouse positions relative to the document\n",
       "    // offset() returns the position of the element relative to the document\n",
       "    var x = e.pageX - $(targ).offset().left;\n",
       "    var y = e.pageY - $(targ).offset().top;\n",
       "\n",
       "    return {\"x\": x, \"y\": y};\n",
       "};\n",
       "\n",
       "/*\n",
       " * return a copy of an object with only non-object keys\n",
       " * we need this to avoid circular references\n",
       " * http://stackoverflow.com/a/24161582/3208463\n",
       " */\n",
       "function simpleKeys (original) {\n",
       "  return Object.keys(original).reduce(function (obj, key) {\n",
       "    if (typeof original[key] !== 'object')\n",
       "        obj[key] = original[key]\n",
       "    return obj;\n",
       "  }, {});\n",
       "}\n",
       "\n",
       "mpl.figure.prototype.mouse_event = function(event, name) {\n",
       "    var canvas_pos = mpl.findpos(event)\n",
       "\n",
       "    if (name === 'button_press')\n",
       "    {\n",
       "        this.canvas.focus();\n",
       "        this.canvas_div.focus();\n",
       "    }\n",
       "\n",
       "    var x = canvas_pos.x * mpl.ratio;\n",
       "    var y = canvas_pos.y * mpl.ratio;\n",
       "\n",
       "    this.send_message(name, {x: x, y: y, button: event.button,\n",
       "                             step: event.step,\n",
       "                             guiEvent: simpleKeys(event)});\n",
       "\n",
       "    /* This prevents the web browser from automatically changing to\n",
       "     * the text insertion cursor when the button is pressed.  We want\n",
       "     * to control all of the cursor setting manually through the\n",
       "     * 'cursor' event from matplotlib */\n",
       "    event.preventDefault();\n",
       "    return false;\n",
       "}\n",
       "\n",
       "mpl.figure.prototype._key_event_extra = function(event, name) {\n",
       "    // Handle any extra behaviour associated with a key event\n",
       "}\n",
       "\n",
       "mpl.figure.prototype.key_event = function(event, name) {\n",
       "\n",
       "    // Prevent repeat events\n",
       "    if (name == 'key_press')\n",
       "    {\n",
       "        if (event.which === this._key)\n",
       "            return;\n",
       "        else\n",
       "            this._key = event.which;\n",
       "    }\n",
       "    if (name == 'key_release')\n",
       "        this._key = null;\n",
       "\n",
       "    var value = '';\n",
       "    if (event.ctrlKey && event.which != 17)\n",
       "        value += \"ctrl+\";\n",
       "    if (event.altKey && event.which != 18)\n",
       "        value += \"alt+\";\n",
       "    if (event.shiftKey && event.which != 16)\n",
       "        value += \"shift+\";\n",
       "\n",
       "    value += 'k';\n",
       "    value += event.which.toString();\n",
       "\n",
       "    this._key_event_extra(event, name);\n",
       "\n",
       "    this.send_message(name, {key: value,\n",
       "                             guiEvent: simpleKeys(event)});\n",
       "    return false;\n",
       "}\n",
       "\n",
       "mpl.figure.prototype.toolbar_button_onclick = function(name) {\n",
       "    if (name == 'download') {\n",
       "        this.handle_save(this, null);\n",
       "    } else {\n",
       "        this.send_message(\"toolbar_button\", {name: name});\n",
       "    }\n",
       "};\n",
       "\n",
       "mpl.figure.prototype.toolbar_button_onmouseover = function(tooltip) {\n",
       "    this.message.textContent = tooltip;\n",
       "};\n",
       "mpl.toolbar_items = [[\"Home\", \"Reset original view\", \"fa fa-home icon-home\", \"home\"], [\"Back\", \"Back to  previous view\", \"fa fa-arrow-left icon-arrow-left\", \"back\"], [\"Forward\", \"Forward to next view\", \"fa fa-arrow-right icon-arrow-right\", \"forward\"], [\"\", \"\", \"\", \"\"], [\"Pan\", \"Pan axes with left mouse, zoom with right\", \"fa fa-arrows icon-move\", \"pan\"], [\"Zoom\", \"Zoom to rectangle\", \"fa fa-square-o icon-check-empty\", \"zoom\"], [\"\", \"\", \"\", \"\"], [\"Download\", \"Download plot\", \"fa fa-floppy-o icon-save\", \"download\"]];\n",
       "\n",
       "mpl.extensions = [\"eps\", \"jpeg\", \"pdf\", \"png\", \"ps\", \"raw\", \"svg\", \"tif\"];\n",
       "\n",
       "mpl.default_extension = \"png\";var comm_websocket_adapter = function(comm) {\n",
       "    // Create a \"websocket\"-like object which calls the given IPython comm\n",
       "    // object with the appropriate methods. Currently this is a non binary\n",
       "    // socket, so there is still some room for performance tuning.\n",
       "    var ws = {};\n",
       "\n",
       "    ws.close = function() {\n",
       "        comm.close()\n",
       "    };\n",
       "    ws.send = function(m) {\n",
       "        //console.log('sending', m);\n",
       "        comm.send(m);\n",
       "    };\n",
       "    // Register the callback with on_msg.\n",
       "    comm.on_msg(function(msg) {\n",
       "        //console.log('receiving', msg['content']['data'], msg);\n",
       "        // Pass the mpl event to the overriden (by mpl) onmessage function.\n",
       "        ws.onmessage(msg['content']['data'])\n",
       "    });\n",
       "    return ws;\n",
       "}\n",
       "\n",
       "mpl.mpl_figure_comm = function(comm, msg) {\n",
       "    // This is the function which gets called when the mpl process\n",
       "    // starts-up an IPython Comm through the \"matplotlib\" channel.\n",
       "\n",
       "    var id = msg.content.data.id;\n",
       "    // Get hold of the div created by the display call when the Comm\n",
       "    // socket was opened in Python.\n",
       "    var element = $(\"#\" + id);\n",
       "    var ws_proxy = comm_websocket_adapter(comm)\n",
       "\n",
       "    function ondownload(figure, format) {\n",
       "        window.open(figure.imageObj.src);\n",
       "    }\n",
       "\n",
       "    var fig = new mpl.figure(id, ws_proxy,\n",
       "                           ondownload,\n",
       "                           element.get(0));\n",
       "\n",
       "    // Call onopen now - mpl needs it, as it is assuming we've passed it a real\n",
       "    // web socket which is closed, not our websocket->open comm proxy.\n",
       "    ws_proxy.onopen();\n",
       "\n",
       "    fig.parent_element = element.get(0);\n",
       "    fig.cell_info = mpl.find_output_cell(\"<div id='\" + id + \"'></div>\");\n",
       "    if (!fig.cell_info) {\n",
       "        console.error(\"Failed to find cell for figure\", id, fig);\n",
       "        return;\n",
       "    }\n",
       "\n",
       "    var output_index = fig.cell_info[2]\n",
       "    var cell = fig.cell_info[0];\n",
       "\n",
       "};\n",
       "\n",
       "mpl.figure.prototype.handle_close = function(fig, msg) {\n",
       "    var width = fig.canvas.width/mpl.ratio\n",
       "    fig.root.unbind('remove')\n",
       "\n",
       "    // Update the output cell to use the data from the current canvas.\n",
       "    fig.push_to_output();\n",
       "    var dataURL = fig.canvas.toDataURL();\n",
       "    // Re-enable the keyboard manager in IPython - without this line, in FF,\n",
       "    // the notebook keyboard shortcuts fail.\n",
       "    IPython.keyboard_manager.enable()\n",
       "    $(fig.parent_element).html('<img src=\"' + dataURL + '\" width=\"' + width + '\">');\n",
       "    fig.close_ws(fig, msg);\n",
       "}\n",
       "\n",
       "mpl.figure.prototype.close_ws = function(fig, msg){\n",
       "    fig.send_message('closing', msg);\n",
       "    // fig.ws.close()\n",
       "}\n",
       "\n",
       "mpl.figure.prototype.push_to_output = function(remove_interactive) {\n",
       "    // Turn the data on the canvas into data in the output cell.\n",
       "    var width = this.canvas.width/mpl.ratio\n",
       "    var dataURL = this.canvas.toDataURL();\n",
       "    this.cell_info[1]['text/html'] = '<img src=\"' + dataURL + '\" width=\"' + width + '\">';\n",
       "}\n",
       "\n",
       "mpl.figure.prototype.updated_canvas_event = function() {\n",
       "    // Tell IPython that the notebook contents must change.\n",
       "    IPython.notebook.set_dirty(true);\n",
       "    this.send_message(\"ack\", {});\n",
       "    var fig = this;\n",
       "    // Wait a second, then push the new image to the DOM so\n",
       "    // that it is saved nicely (might be nice to debounce this).\n",
       "    setTimeout(function () { fig.push_to_output() }, 1000);\n",
       "}\n",
       "\n",
       "mpl.figure.prototype._init_toolbar = function() {\n",
       "    var fig = this;\n",
       "\n",
       "    var nav_element = $('<div/>')\n",
       "    nav_element.attr('style', 'width: 100%');\n",
       "    this.root.append(nav_element);\n",
       "\n",
       "    // Define a callback function for later on.\n",
       "    function toolbar_event(event) {\n",
       "        return fig.toolbar_button_onclick(event['data']);\n",
       "    }\n",
       "    function toolbar_mouse_event(event) {\n",
       "        return fig.toolbar_button_onmouseover(event['data']);\n",
       "    }\n",
       "\n",
       "    for(var toolbar_ind in mpl.toolbar_items){\n",
       "        var name = mpl.toolbar_items[toolbar_ind][0];\n",
       "        var tooltip = mpl.toolbar_items[toolbar_ind][1];\n",
       "        var image = mpl.toolbar_items[toolbar_ind][2];\n",
       "        var method_name = mpl.toolbar_items[toolbar_ind][3];\n",
       "\n",
       "        if (!name) { continue; };\n",
       "\n",
       "        var button = $('<button class=\"btn btn-default\" href=\"#\" title=\"' + name + '\"><i class=\"fa ' + image + ' fa-lg\"></i></button>');\n",
       "        button.click(method_name, toolbar_event);\n",
       "        button.mouseover(tooltip, toolbar_mouse_event);\n",
       "        nav_element.append(button);\n",
       "    }\n",
       "\n",
       "    // Add the status bar.\n",
       "    var status_bar = $('<span class=\"mpl-message\" style=\"text-align:right; float: right;\"/>');\n",
       "    nav_element.append(status_bar);\n",
       "    this.message = status_bar[0];\n",
       "\n",
       "    // Add the close button to the window.\n",
       "    var buttongrp = $('<div class=\"btn-group inline pull-right\"></div>');\n",
       "    var button = $('<button class=\"btn btn-mini btn-primary\" href=\"#\" title=\"Stop Interaction\"><i class=\"fa fa-power-off icon-remove icon-large\"></i></button>');\n",
       "    button.click(function (evt) { fig.handle_close(fig, {}); } );\n",
       "    button.mouseover('Stop Interaction', toolbar_mouse_event);\n",
       "    buttongrp.append(button);\n",
       "    var titlebar = this.root.find($('.ui-dialog-titlebar'));\n",
       "    titlebar.prepend(buttongrp);\n",
       "}\n",
       "\n",
       "mpl.figure.prototype._root_extra_style = function(el){\n",
       "    var fig = this\n",
       "    el.on(\"remove\", function(){\n",
       "\tfig.close_ws(fig, {});\n",
       "    });\n",
       "}\n",
       "\n",
       "mpl.figure.prototype._canvas_extra_style = function(el){\n",
       "    // this is important to make the div 'focusable\n",
       "    el.attr('tabindex', 0)\n",
       "    // reach out to IPython and tell the keyboard manager to turn it's self\n",
       "    // off when our div gets focus\n",
       "\n",
       "    // location in version 3\n",
       "    if (IPython.notebook.keyboard_manager) {\n",
       "        IPython.notebook.keyboard_manager.register_events(el);\n",
       "    }\n",
       "    else {\n",
       "        // location in version 2\n",
       "        IPython.keyboard_manager.register_events(el);\n",
       "    }\n",
       "\n",
       "}\n",
       "\n",
       "mpl.figure.prototype._key_event_extra = function(event, name) {\n",
       "    var manager = IPython.notebook.keyboard_manager;\n",
       "    if (!manager)\n",
       "        manager = IPython.keyboard_manager;\n",
       "\n",
       "    // Check for shift+enter\n",
       "    if (event.shiftKey && event.which == 13) {\n",
       "        this.canvas_div.blur();\n",
       "        // select the cell after this one\n",
       "        var index = IPython.notebook.find_cell_index(this.cell_info[0]);\n",
       "        IPython.notebook.select(index + 1);\n",
       "    }\n",
       "}\n",
       "\n",
       "mpl.figure.prototype.handle_save = function(fig, msg) {\n",
       "    fig.ondownload(fig, null);\n",
       "}\n",
       "\n",
       "\n",
       "mpl.find_output_cell = function(html_output) {\n",
       "    // Return the cell and output element which can be found *uniquely* in the notebook.\n",
       "    // Note - this is a bit hacky, but it is done because the \"notebook_saving.Notebook\"\n",
       "    // IPython event is triggered only after the cells have been serialised, which for\n",
       "    // our purposes (turning an active figure into a static one), is too late.\n",
       "    var cells = IPython.notebook.get_cells();\n",
       "    var ncells = cells.length;\n",
       "    for (var i=0; i<ncells; i++) {\n",
       "        var cell = cells[i];\n",
       "        if (cell.cell_type === 'code'){\n",
       "            for (var j=0; j<cell.output_area.outputs.length; j++) {\n",
       "                var data = cell.output_area.outputs[j];\n",
       "                if (data.data) {\n",
       "                    // IPython >= 3 moved mimebundle to data attribute of output\n",
       "                    data = data.data;\n",
       "                }\n",
       "                if (data['text/html'] == html_output) {\n",
       "                    return [cell, data, j];\n",
       "                }\n",
       "            }\n",
       "        }\n",
       "    }\n",
       "}\n",
       "\n",
       "// Register the function which deals with the matplotlib target/channel.\n",
       "// The kernel may be null if the page has been refreshed.\n",
       "if (IPython.notebook.kernel != null) {\n",
       "    IPython.notebook.kernel.comm_manager.register_target('matplotlib', mpl.mpl_figure_comm);\n",
       "}\n"
      ],
      "text/plain": [
       "<IPython.core.display.Javascript object>"
      ]
     },
     "metadata": {},
     "output_type": "display_data"
    },
    {
     "data": {
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\" width=\"600\">"
      ],
      "text/plain": [
       "<IPython.core.display.HTML object>"
      ]
     },
     "metadata": {},
     "output_type": "display_data"
    }
   ],
   "source": [
    "plt.title('ROIs per well of %s' % plate.id)\n",
    "plt.pcolor(total)\n",
    "plt.colorbar()\n",
    "plt.show()"
   ]
  },
  {
   "cell_type": "markdown",
   "metadata": {
    "collapsed": true
   },
   "source": [
    "### License (BSD 2-Clause)¶\n",
    "\n",
    "Copyright (C) 2016-2021 University of Dundee. All Rights Reserved.\n",
    "\n",
    "Redistribution and use in source and binary forms, with or without modification, are permitted provided that the following conditions are met:\n",
    "\n",
    "Redistributions of source code must retain the above copyright notice, this list of conditions and the following disclaimer. Redistributions in binary form must reproduce the above copyright notice, this list of conditions and the following disclaimer in the documentation and/or other materials provided with the distribution. THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS \"AS IS\" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT OWNER OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE."
   ]
  }
 ],
 "metadata": {
  "kernelspec": {
   "display_name": "Python 3",
   "language": "python",
   "name": "python3"
  },
  "language_info": {
   "codemirror_mode": {
    "name": "ipython",
    "version": 3
   },
   "file_extension": ".py",
   "mimetype": "text/x-python",
   "name": "python",
   "nbconvert_exporter": "python",
   "pygments_lexer": "ipython3",
   "version": "3.7.10"
  }
 },
 "nbformat": 4,
 "nbformat_minor": 2
}