Error running 1 of 2 channels for Step 3

[edelaserna]

Hi! I am trying to generate the surface mesh for one channel of my image stack. I have loaded both channel stacks as separate channels in a movie. When I start at Step 3 and only select 1 of the 2 channels, I get the following error report:

--Step 3: Mesh                                                                                                                                                                                   
                                                                                                                                                                                                 
Caused by:                                                                                                                                                                                       
Intermediate brace '{}' indexing produced a comma-separated list with 0 values, but it must produce a single value when followed by subsequent indexing operations.   

I do not have this issue when I run Step 3 with a movie containing only 1 channel. I am making sure to check "use deconvolved", so that does not seem to be the issue. Is there a step to handling multi-channel movies that I might be missing that would generate this indexing error? Thanks!

[edelaserna]

To try to get around this channel indexing issue, I used the "Prepare" function in the GUI to create the movie. I was able to load in my 2 channels successfully and view them together, however when I tried to open the Morphology3D package with the movie, I get this error:

-- Morphology3D initialization                                                                                                                                                   
                                                                                                                                                                                 
Error using Morphology3DPackage/sanityCheck (line 47)                                                                                                                            
Missing MovieData is not 3D!! The MovieData is not 3D! is necessary to analyze 3D Cells. Please edit the movie and fill the MovieData is not 3D!.                                
                                                                                                                                                                                 
Error in packageGUI_OpeningFcn (line 184)                                                                                                                                        
        userData.package(i).sanityCheck(true,'all');                                                                                                                             
                                                                                                                                                                                 
Error in gui_mainfcn (line 220)                                                                                                                                                  
    feval(gui_State.gui_OpeningFcn, gui_hFigure, [], guidata(gui_hFigure), varargin{:});                                                                                         
                                                                                                                                                                                 
Error in packageGUI (line 61)                                                                                                                                                    
    gui_mainfcn(gui_State, varargin{:});                                                                                                                                         
                                                                                                                                                                                 
Error in movieSelectorGUI>pushbutton_done_Callback (line 259)                                                                                                                    
packageGUI(selectedPackage,userData.(field),...                                                                                                                                  
                                                                                                                                                                                 
Error in gui_mainfcn (line 95)                                                                                                                                                   
        feval(varargin{:});                                                                                                                                                      
                                                                                                                                                                                 
Error in movieSelectorGUI (line 61)                                                                                                                                              
    gui_mainfcn(gui_State, varargin{:});                                                                                                                                         
                                                                                                                                                                                 
Error in matlab.graphics.internal.figfile.FigFile/read>@(hObject,eventdata)movieSelectorGUI('pushbutton_done_Callback',hObject,eventdata,guidata(hObject))                       
Please solve the above problems before continuing.                                                                                                                               
                                                                                                                                                                                 
The movie(s) could not be processed.            

Something seems wrong here, because I'm specifying the pixel size z of my image stacks and I'm also able to view the movie with the viewer tool and confirm it is 3D. Is it possible that the sanityCheck step is too restrictive and that's what is causing this problem?

[DanuserLab]

Thank you for reporting the issue.

We were unable to reproduce the error when running one of 2 channels of a movie for step 3. If you have any further questions or need additional assistance, please let us know.

Best regards.