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Integrating SCTransform-normalised data with LIGER #277

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lucygarner opened this issue Nov 1, 2022 · 1 comment
Open

Integrating SCTransform-normalised data with LIGER #277

lucygarner opened this issue Nov 1, 2022 · 1 comment

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@lucygarner
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lucygarner commented Nov 1, 2022

Related to #161, is it still necessary to manually ensure that the Pearson residuals are non-negative in order to use SCTransform-normalised data for LIGER?

Do I just start from RunOptimizeALS after running SCTransform? And would I run SCTransform with all batches combined or for each batch separately?

Is this tutorial up-to-date for the current version of LIGER?
http://htmlpreview.github.io/?https://github.com/satijalab/seurat-wrappers/blob/master/docs/liger.html

Thanks!

@cblazier
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cblazier commented Feb 3, 2023

I would also like to know whether I can use SCTransform on my data before starting with the Seurat-to-LIGER pipeline, and whether I should do it on samples separately or combined!

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