libfastk.h

/*******************************************************************************************
 *
 *  C library routines to access and operate upon FastK histogram, k-mer tables, and profiles
 *
 *  Author:  Gene Myers
 *  Date  :  November 2020
 *
 *******************************************************************************************/

#ifndef _LIBFASTK
#define _LIBFASTK

#include <stdio.h>
#include <stdlib.h>
#include <string.h>
#include <ctype.h>
#include <stdint.h>
#include <math.h>
#include <dirent.h>
#include <fcntl.h>
#include <sys/types.h>
#include <sys/uio.h>
#include <unistd.h>
#include <errno.h>

#include "gene_core.h"

  //  HISTOGRAM

typedef struct
  { int    kmer;    //  Histogram is for k-mers of this length
    int    unique;  // 1 => count  of unique k-mers, 0 => count of k-mer instances
    int    low;     //  Histogram is for range [low,hgh]
    int    high;
    int64 *hist;    //  hist[i] for i in [low,high] = # of k-mers occuring i times
  } Histogram;

Histogram *Load_Histogram(char *name);
void       Modify_Histogram(Histogram *H, int low, int high, int unique);
int        Write_Histogram(char *name, Histogram *H);
void       Free_Histogram(Histogram *H);


  //  K-MER TABLE

typedef struct
  { int     kmer;         //  Kmer length
    int     minval;       //  The minimum count of a k-mer in the table
    int64   nels;         //  # of unique, sorted k-mers in the table

    void   *private[7];   //  Private fields
  } Kmer_Table;

Kmer_Table *Load_Kmer_Table(char *name, int cut_off);
void        Free_Kmer_Table(Kmer_Table *T);

char       *Fetch_Kmer(Kmer_Table *T, int64 i, char *seq);
int         Fetch_Count(Kmer_Table *T, int64 i);

int64       Find_Kmer(Kmer_Table *T, char *kseq);


  //  K-MER STREAM

typedef struct
  { int    kmer;       //  Kmer length
    int    minval;     //  The minimum count of a k-mer in the stream
    int64  nels;       //  # of elements in entire table
                    // Current position
    int64  cidx;       //  current element index
    uint8 *csuf;       //  current element suffix
    int    cpre;       //  current element prefix
                    // Other useful parameters
    int    ibyte;      //  # of bytes in prefix
    int    kbyte;      //  Kmer encoding in bytes
    int    tbyte;      //  Kmer+count entry in bytes
    int    hbyte;      //  Kmer suffix in bytes (= kbyte - ibyte)
    int    pbyte;      //  Kmer,count suffix in bytes (= tbyte - ibyte)

    void  *private[10]; //  Private fields
  } Kmer_Stream;

Kmer_Stream *Open_Kmer_Stream(char *name);
Kmer_Stream *Clone_Kmer_Stream(Kmer_Stream *S);
void         Free_Kmer_Stream(Kmer_Stream *S);

void         First_Kmer_Entry(Kmer_Stream *S);
void         Next_Kmer_Entry(Kmer_Stream *S);

char        *Current_Kmer(Kmer_Stream *S, char *seq);
int          Current_Count(Kmer_Stream *S);
uint8       *Current_Entry(Kmer_Stream *S, uint8 *seq);

void         GoTo_Kmer_Index(Kmer_Stream *S, int64 idx);
int          GoTo_Kmer_String(Kmer_Stream *S, char *seq);
int          GoTo_Kmer_Entry(Kmer_Stream *S, uint8 *entry);


  //  PROFILES

typedef struct
  { int    kmer;     //  Kmer length
    int    nparts;   //  # of threads/parts for the profiles
    int    nreads;   //  total # of reads in data set
    int64 *nbase;    //  nbase[i] for i in [0,nparts) = id of last read in part i + 1
    int64 *index;    //  index[i] for i in [0,nreads) = offset in relevant part of
                     //    compressed profile for read i
    void  *private[4]; // Private fields
  } Profile_Index;

Profile_Index *Open_Profiles(char *name);
Profile_Index *Clone_Profiles(Profile_Index *P);

void Free_Profiles(Profile_Index *P);

int64 Fetch_Profile(Profile_Index *P, int64 id, int64 plen, uint16 *profile);

#endif // _LIBFASTK