HHblits failed

[skhatri007]

I am getting the following error while running alphafold :

Started HHblits query
I0811 15:48:12.553200 22849205610304 utils.py:40] Finished HHblits query in 0.134 seconds
E0811 15:48:12.553327 22849205610304 hhblits.py:138] HHblits failed. HHblits stderr begin:
E0811 15:48:12.553383 22849205610304 hhblits.py:142] HHblits stderr end
Traceback (most recent call last):
File "/app/alphafold/run_alphafold.py", line 432, in
app.run(main)
File "/opt/conda/lib/python3.8/site-packages/absl/app.py", line 312, in run
_run_main(main, args)
File "/opt/conda/lib/python3.8/site-packages/absl/app.py", line 258, in _run_main
sys.exit(main(argv))
File "/app/alphafold/run_alphafold.py", line 408, in main
predict_structure(
File "/app/alphafold/run_alphafold.py", line 172, in predict_structure
feature_dict = data_pipeline.process(
File "/app/alphafold/alphafold/data/pipeline_multimer.py", line 264, in process
chain_features = self._process_single_chain(
File "/app/alphafold/alphafold/data/pipeline_multimer.py", line 212, in _process_single_chain
chain_features = self._monomer_data_pipeline.process(
File "/app/alphafold/alphafold/data/pipeline.py", line 215, in process
hhblits_bfd_uniref_result = run_msa_tool(
File "/app/alphafold/alphafold/data/pipeline.py", line 96, in run_msa_tool
result = msa_runner.query(input_fasta_path)[0]
File "/app/alphafold/alphafold/data/tools/hhblits.py", line 143, in query
raise RuntimeError('HHblits failed\nstdout:\n%s\n\nstderr:\n%s\n' % (
RuntimeError: HHblits failed
stdout:

stderr:

I am giving enough memmory and cpu and tried changing a lot of parameters but nothing is helping .

Resources:

#SBATCH --job-name=ATG7-ATG3-LC3B
#SBATCH --output=ATG7-ATG3-LC3B.out
#SBATCH --cpus-per-task=64
#SBATCH --partition=dgx
#SBATCH --gres=gpu:4
#SBATCH --mem=326G

command:

export ALPHAFOLD_DIR="/lustre/skhatri/alphafold_singularity/alphafold-2.3.1"

DOWNLOAD_DIR=/home/skhatri/alphafold/D_B

python3 run_singularity.py --use_gpu --data_dir=$DOWNLOAD_DIR --fasta_paths=test.fasta --max_template_date=2023-06-14 --output_dir=/lustre/skhatri/alphafold_singularity/alphafold-2.3.1/results --model_preset=multimer

Kindly help with this error.