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inconsistency using fragment or whole protein #341

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twaksman001 opened this issue Feb 27, 2023 · 0 comments
Open

inconsistency using fragment or whole protein #341

twaksman001 opened this issue Feb 27, 2023 · 0 comments

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@twaksman001
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I used HHBlits via the MPI website

If I submit whole protein sequence - 10000 matches, mostly kinase proteins, and the alignment is to a subsection of the query.

If I resubmit this subsection, <50 matches and no kinases.

Please can you explain how this happens?

Protein sequence: NPLCPYMNRGNPNLLPQQKSSTTQGDQVYAPSMPQFSMEGNRQMYPMPPPMMPPPPMMQGFQPPFPFNPMNQQQLSMLLMSSYARAMALAQLMNMQNRFQFYQPNQYSDPAINNYSSMPATLPQQPQVDLQNNLKTEMSPEQEKQGEPGEPRESELETSQQQGELQQQEEPLNSGEGQETEVSGEREEPETRDQDILEKRKMQRALDIEIERRERLQKRKAEENELERQQKEMERMLREEEEMKRQEEETKRQILEEERKEREEKEKDIKLRQEEELRFKKIEAEKKKRNQVNNKPQGTSVRRQRKQKHTKKTVTEDIIERL

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