Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

error in using zUMIs to processing smartseq3 data #391

Open
GGboy-Zzz opened this issue Feb 23, 2024 · 0 comments
Open

error in using zUMIs to processing smartseq3 data #391

GGboy-Zzz opened this issue Feb 23, 2024 · 0 comments

Comments

@GGboy-Zzz
Copy link

Thank you for your awesome software! I got several errors in processing my smartseq3 data, and I have scanned some related_issues, yet it's not solved my problem. First, because several cells have the same index, I merged demultiplexed fastq files by script 'merge_demultiplexed_fastq.R'. Then I run zumis.sh by slurm_script, below are yaml file and error log (surprising, its size is 2.5GB),
smartseq3.yaml
error_log
image
The run would produce results files, but the 'feature.pdf' showed there was too much unused barcodes. I think this is wrong. I refered to the issuse310, but it wouldnt produce result if I didnt offer index_fastq files. Can you give me some kindful help to solve it? I will be deeply grateful. Thanks! @cziegenhain
image

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

1 participant