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I would like to addModuleScore to my R object.
If I use my default assay of integrated, then I get errors that my features are not present in my object
If I switch to RNA assay as my default, I get the following error: Error in GetAssayData(): ! GetAssayData doesn't work for multiple layers in v5 assay. Run rlang::last_trace() to see where the error occurred.
I've tried the function on another R object and it works, however I cannot get it to work with my current object and am unsure why.
It works for this one:
does not work for this one:
I also am not sure why I have layers present like "scale.data.mock2.1" in my monkeypox.integrated object, this becomes an error when I attempt to access the data at:
rownames(monkeypox.integrated@assays$RNA$counts)
where it makes me pick either counts.mock2.1 or counts.mkpx2.2, but am unsure which one to use in the context of calculating DEGs and running string DB. How do you know when to use which?
The text was updated successfully, but these errors were encountered:
jimena2
changed the title
addModuleScore error and general errors please send help
addModuleScore error and general errors
Apr 30, 2024
I would like to addModuleScore to my R object.
If I use my default assay of integrated, then I get errors that my features are not present in my object
If I switch to RNA assay as my default, I get the following error:
Error in
GetAssayData(): ! GetAssayData doesn't work for multiple layers in v5 assay. Run
rlang::last_trace()to see where the error occurred.
I've tried the function on another R object and it works, however I cannot get it to work with my current object and am unsure why.
It works for this one:
does not work for this one:
I also am not sure why I have layers present like "scale.data.mock2.1" in my monkeypox.integrated object, this becomes an error when I attempt to access the data at:
rownames(monkeypox.integrated@assays$RNA$counts)
where it makes me pick either counts.mock2.1 or counts.mkpx2.2, but am unsure which one to use in the context of calculating DEGs and running string DB. How do you know when to use which?
The text was updated successfully, but these errors were encountered: