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Yes I have been experiencing a similar issue in the work I was conducting. Hoping for a response |
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Hello,
I am working on a dataset with multiple samples that have different stimulated timepoints and I am trying to run SCTransform on each object/sample individually prior to performing integration.
I was wondering about the use of the split function seen below that is used in Introduction to scRNA-seq integration vignette.
It is used prior to running SCTransform and CCA integration.
dataset["RNA"]] <- split(dataset[["RNA"]], f = dataset$timepoint)
Does this function split the dataset, allowing one to run SCTransform on each layer individually. Or would I still have to run SCTransform on each sample separately and then merge ?
Any help/guidance is appreciated!
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