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Hi,
I performed canu assembly and trying to circularize the 1.54Mb contiguous with following one of the parameters:
circlator all --assembler canu --data_type pacbio-corrected --bwa_opts "-x ont2d" ---b2r_length_cutoff 30000 merge_min_id 85 --merge_breaklen 1000 1.54mbcontig.fa p.correctedReads.fasta.gz output_cir_85_1000
I tried different combinations but all of them are not working. Please suggest what parameters I can add to make it circular. Should I have to polish it before circularization?
Thank You
The text was updated successfully, but these errors were encountered:
Hi,
I performed canu assembly and trying to circularize the 1.54Mb contiguous with following one of the parameters:
circlator all --assembler canu --data_type pacbio-corrected --bwa_opts "-x ont2d" ---b2r_length_cutoff 30000 merge_min_id 85 --merge_breaklen 1000 1.54mbcontig.fa p.correctedReads.fasta.gz output_cir_85_1000
I tried different combinations but all of them are not working. Please suggest what parameters I can add to make it circular. Should I have to polish it before circularization?
Thank You
The text was updated successfully, but these errors were encountered: