You signed in with another tab or window. Reload to refresh your session.You signed out in another tab or window. Reload to refresh your session.You switched accounts on another tab or window. Reload to refresh your session.Dismiss alert
Hi,
I am using Rolling Circle Amplification technique which produces very long amplicons. After amplification, I fragment these products to approximately 1 genomelength and sequence this by MinION. I assembled the data with Canu, which ends up in an assembly of 6 times the genome length, since there were multiple reads >2 times the total length.
By using Circlator, I was able to reduce the assembly length to 3 times the genome.
Is it possible to reduce this to 1? Or do I have to manually cut my reads to succeed?
Thanks, Tim
The text was updated successfully, but these errors were encountered:
Hi,
I am using Rolling Circle Amplification technique which produces very long amplicons. After amplification, I fragment these products to approximately 1 genomelength and sequence this by MinION. I assembled the data with Canu, which ends up in an assembly of 6 times the genome length, since there were multiple reads >2 times the total length.
By using Circlator, I was able to reduce the assembly length to 3 times the genome.
Is it possible to reduce this to 1? Or do I have to manually cut my reads to succeed?
Thanks, Tim
The text was updated successfully, but these errors were encountered: