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After running circlator reduction of 6 genomes in assembly to 3. #150

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severst opened this issue Mar 29, 2019 · 0 comments
Open

After running circlator reduction of 6 genomes in assembly to 3. #150

severst opened this issue Mar 29, 2019 · 0 comments

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@severst
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severst commented Mar 29, 2019

Hi,
I am using Rolling Circle Amplification technique which produces very long amplicons. After amplification, I fragment these products to approximately 1 genomelength and sequence this by MinION. I assembled the data with Canu, which ends up in an assembly of 6 times the genome length, since there were multiple reads >2 times the total length.
By using Circlator, I was able to reduce the assembly length to 3 times the genome.
Is it possible to reduce this to 1? Or do I have to manually cut my reads to succeed?
Thanks, Tim

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