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Finder generates no files and fails #75

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ereyred opened this issue Mar 20, 2023 · 1 comment
Open

Finder generates no files and fails #75

ereyred opened this issue Mar 20, 2023 · 1 comment

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@ereyred
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ereyred commented Mar 20, 2023

Hello, I'm running Finder from local installation, not Docker. I'm running with PE RNAseq.fq but no protein file. It makes the necessary output directories and protein dummy file in temp but no other output and it gives me the following error:

Status: Downloaded newer image for sagnikbanerjee15/finder:1.1.0
docker.io/sagnikbanerjee15/finder:1.1.0
done
Traceback (most recent call last):
File "/softwares/FINDER/Finder/finder", line 688, in
main()
File "/softwares/FINDER/Finder/finder", line 637, in main
readMetaDataFile( options, logger_proxy, logging_mutex )
File "/softwares/FINDER/Finder/scripts/fileReadWriteOperations.py", line 138, in readMetaDataFile
small_rna_samples[condition][Run] = {"bioproject":BioProject,
KeyError: ''

The progress.log out put is:
2023-03-19 23:53:40,489 - finder - INFO - Running command - perl
/Finder_v1.1.0/gmes_linux_64//change_path_in_perl_scripts.pl "/usr/bin/env perl"
2023-03-19 23:53:40,916 - finder - INFO - Software paths have been set
2023-03-19 23:53:41,498 - finder - INFO - Generating STAR index
2023-03-19 23:53:41,909 - finder - INFO - Running cmd - STAR --runThreadN 20 --runMode genomeGenerate --genomeDir
/Finder_v1.1.0/output/indices/star_index_without_transcriptome --genomeFastaFiles /Finder_v1.1.0/output/Genome.fasta.masked --genomeChrBinNbits 18 --genomeSAindexNbases 11.740410536216816 --outFileNamePrefix
/Finder_v1.1.0/output/alignments/indexGenome > /Finder_v1.1.0/output/indices/star_index_without_transcriptome.output 2> /Finder_v1.1.0/output/indices/star_index_without_transcriptome.error
2023-03-19 23:53:56,972 - finder - INFO - STAR index generation complete
2023-03-19 23:53:56,973 - finder - INFO - Generating OLego index
2023-03-19 23:53:56,974 - finder - INFO - Running cmd - olegoindex -p
/Finder_v1.1.0/output/indices/olego_index /Finder_v1.1.0/Genome.fasta.masked > /Finder_v1.1.0/output/indices_olego_index.output 2> /Finder_v1.1.0/output/indices_olego_index.error
2023-03-19 23:54:59,284 - finder - INFO - OLego index built
2023-03-19 23:54:59,286 - finder - INFO - Adding dummy file since no protein evidence was provided
2023-03-19 23:54:59,286 - finder - INFO - validateCommandLineArguments execution successful

My metadata file looks like this:
BioProject SRA Accession Tissues Description Date Read Length (bp) Ended RNA Seq process Location
Name S1_8 na PE 1 1 /dir/Finder_v1.1.0/

Thanks for your help!
E

@sagnikbanerjee15
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Hello @ereyred,

Thank you for your interest in finder. We are aware of the issues in the previous version but we have decided to move forward with new improvements. As of now, we do not have any plans of fixing the issues in the previous version since they will be taken cared of in the new version.

Thank you.

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