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parameters for nanopore Q20 reads #267

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bernardo1963 opened this issue Apr 19, 2024 · 1 comment
Open

parameters for nanopore Q20 reads #267

bernardo1963 opened this issue Apr 19, 2024 · 1 comment

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@bernardo1963
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Dear Dr Jue Ruan,
I am using wtdbg2 with the new nanopore Q20 reads. I tried setting -x ccs , but got a very fragmented and incomplete assembly.
May you please suggest which parameters should I try? Should I correct the reads (eg, with Ratatosk) before assembly?
Yours,
Bernardo

@ruanjue
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ruanjue commented Apr 22, 2024

I haven't tested on nanopore Q20 data. Maybe you need to polish them first, or try -x ont or -x sq.

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