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No such file: assembly.events #252

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ykc21e8 opened this issue Dec 15, 2022 · 4 comments
Open

No such file: assembly.events #252

ykc21e8 opened this issue Dec 15, 2022 · 4 comments

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@ykc21e8
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ykc21e8 commented Dec 15, 2022

I initiate my runs with
wtdbg2 -x ont -g 4.8m -t 6 -i *fastq.gz -f -o ./wtdbg2_assembly/assembly

But it gives
[Fri Dec 16 10:58:37 2022] loading reads 226756 reads [Fri Dec 16 10:58:51 2022] filtering from 226756 reads (>=5000 bp), 2071321570 bp. Try selecting 240000000 bp [Fri Dec 16 10:58:51 2022] Done, 8078 reads (>=5000 bp), 240001280 bp, 937428 bins ** PROC_STAT(0) **: real 14.218 sec, user 24.300 sec, sys 2.280 sec, maxrss 976392.0 kB, maxvsize 3365996.0 kB [Fri Dec 16 10:58:51 2022] Set --edge-cov to 3 KEY PARAMETERS: -k 15 -p 0 -K 1000.049988 -A -S 2.000000 -s 0.050000 -g 4800000 -X 50.000000 -e 3 -L 5000 Cannot open file for write: ./wtdbg2_assembly/assembly.events No such file or directory

I don't understand where assembly.events is coming from.
How do I solve this?

@ruanjue
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ruanjue commented Dec 16, 2022

wtdbg2 tried to write log to <prefix>.events, but cannot write this file.

@ykc21e8
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ykc21e8 commented Dec 16, 2022

But I don't have a <prefix>.events file, why is it doing this? Is it supposed to generate this file?

@ruanjue
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ruanjue commented Dec 16, 2022

Here, <prefix> = "./wtdbg2_assembly/assembly". wtdbg2 cannot create or cannot open <prefix>.events. Check your file system first.

@ykc21e8
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ykc21e8 commented Dec 16, 2022

I'm really sorry, I am a beginner, I have no solution for this

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