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Custom adapters cannot be found when binning but can be found using trimming function #101

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v1tazhan9 opened this issue Feb 28, 2024 · 0 comments

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@v1tazhan9
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Hi! I input custom adapter using adapter.py, these adapters can be found if i use -o function for trimming
image
"30,139 / 32,299 reads had adapters trimmed from their end (2,444,748 bp removed)"

but when I use - b function to bin, and it says
"Error: no barcodes were found, so Porechop cannot perform barcode demultiplexing"
i try to set the threshold really really low just to try and get nothing, tried verbosity for debugging and nothing

here's a history:
% porechop -i /Users/vita/Desktop/GladLab/Nanopore_DEPC_data/R1_A_probed/fastq_pass/R1_A_probed.fastq -b /Users/vita/Desktop/GladLab/Nanopore_DEPC_data/porechop/bin --barcode_threshold 20 --barcode_diff 1 --verbosity 3

Loading reads
/Users/vita/Desktop/GladLab/Nanopore_DEPC_data/R1_A_probed/fastq_pass/R1_A_probed.fastq
28,518 reads loaded

Looking for known adapter sets
10,000 / 10,000 (100.0%)
Error: no barcodes were found, so Porechop cannot perform barcode demultiplexing

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