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Dockerfile
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FROM reslp/mamba:0.21.1
RUN conda config --add channels defaults && \
conda config --add channels bioconda && \
conda config --add channels conda-forge && \
#mamba install -y funannotate=1.8.11 "python>=3.6,<3.9" "augustus=3.4" "trinity==2.8.5" "evidencemodeler==1.1.1" "pasa==2.4.1" "codingquarry==2.0" "perl=5.26.2" "diamond=2.0.7"
mamba install -y funannotate=1.8.13 "python>=3.6,<3.9" glimmerhmm=3.0.4=pl526hf484d3e_3 augustus=3.4.0
# some of the mamba packages need to be version controlled for the set PATH variables to be correct. See below.
WORKDIR /software
RUN apt-get --allow-releaseinfo-change update && apt-get install -y build-essential
####RepeatModeler - several dependcies needed for that
# RECON
RUN wget http://www.repeatmasker.org/RepeatModeler/RECON-1.08.tar.gz && \
tar xvfz RECON-1.08.tar.gz && \
cd RECON-1.08/src && \
make && make install && \
sed -i -e 's#\$path = \"\";#\$path = \"/software/RECON-1.08/bin\";#' /software/RECON-1.08/scripts/recon.pl
# RepeatScout
RUN wget http://www.repeatmasker.org/RepeatScout-1.0.6.tar.gz && \
tar xvfz RepeatScout-1.0.6.tar.gz && \
cd RepeatScout-1.0.6 && \
make && \
cp RepeatScout build_lmer_table /usr/local/bin
# TRF
RUN wget http://tandem.bu.edu/trf/downloads/trf409.linux64 && \
chmod +x trf409.linux64 && \
mv trf409.linux64 /usr/local/bin/trf
# rmblast
RUN wget http://www.repeatmasker.org/rmblast-2.9.0+-p2-x64-linux.tar.gz && \
tar xvfz rmblast-2.9.0+-p2-x64-linux.tar.gz
#Repeatmasker
# fixing the perl paths is from: https://github.com/chrishah/maker-docker/blob/master/repeatmasker/Dockerfile
RUN wget http://www.repeatmasker.org/RepeatMasker-open-4-0-7.tar.gz && \
tar xvfz RepeatMasker-open-4-0-7.tar.gz && \
sed -i -e 's#TRF_PRGM = ""#TRF_PRGM = \"/usr/local/bin/trf\"#g' RepeatMasker/RepeatMaskerConfig.tmpl && \
sed -i -e 's#DEFAULT_SEARCH_ENGINE = \"crossmatch\"#DEFAULT_SEARCH_ENGINE = \"ncbi\"#g' RepeatMasker/RepeatMaskerConfig.tmpl && \
sed -i -e 's#RMBLAST_DIR = \"/usr/local/rmblast\"#RMBLAST_DIR = \"/software/rmblast-2.9.0-p2/bin\"#g' RepeatMasker/RepeatMaskerConfig.tmpl && \
sed -i -e 's#REPEATMASKER_DIR = \"\$FindBin::RealBin\"#REPEATMASKER_DIR = \"/software/RepeatMasker\"#g' RepeatMasker/RepeatMaskerConfig.tmpl && \
cp RepeatMasker/RepeatMaskerConfig.tmpl RepeatMasker/RepeatMaskerConfig.pm && \
perl -i -0pe 's/^#\!.*perl.*/#\!\/usr\/bin\/env perl/g' \
RepeatMasker/RepeatMasker \
RepeatMasker/DateRepeats \
RepeatMasker/ProcessRepeats \
RepeatMasker/RepeatProteinMask \
RepeatMasker/DupMasker \
RepeatMasker/util/queryRepeatDatabase.pl \
RepeatMasker/util/queryTaxonomyDatabase.pl \
RepeatMasker/util/rmOutToGFF3.pl \
RepeatMasker/util/rmToUCSCTables.pl
# uses the method by chrishah (https://github.com/chrishah/maker-docker/tree/master/repeatmasker) to build repeatmasker librariers without interactive config.
ADD rebuild /software/RepeatMasker
RUN perl /software/RepeatMasker/rebuild
#Repeatmodeler
# fixing the perl paths is from: https://github.com/chrishah/maker-docker/blob/master/repeatmasker/Dockerfile
RUN wget http://www.repeatmasker.org/RepeatModeler/RepeatModeler-open-1.0.11.tar.gz && \
tar xvfz RepeatModeler-open-1.0.11.tar.gz && \
cd RepeatModeler-open-1.0.11 && \
perl -i -0pe 's/^#\!.*/#\!\/usr\/bin\/env perl/g' \
configure \
BuildDatabase \
Refiner \
RepeatClassifier \
RepeatModeler \
TRFMask \
util/dfamConsensusTool.pl \
util/renameIds.pl \
util/viewMSA.pl && \
sed -i -e 's#RECON_DIR = \"/usr/local/bin\"#RECON_DIR = \"/software/RECON-1.08/bin\"#g' RepModelConfig.pm.tmpl && \
sed -i -e 's#RSCOUT_DIR = \"/usr/local/bin/\"#RSCOUT_DIR = \"/software/RepeatScout-1.0.6\"#g' RepModelConfig.pm.tmpl && \
sed -i -e 's#RMBLAST_DIR = \"/usr/local/rmblast\"#RMBLAST_DIR = \"/software/rmblast-2.9.0-p2/bin\"#g' RepModelConfig.pm.tmpl && \
sed -i -e 's#REPEATMASKER_DIR = \"/usr/local/RepeatMasker\"#REPEATMASKER_DIR = \"/software/RepeatMasker\"#g' RepModelConfig.pm.tmpl && \
cp RepModelConfig.pm.tmpl RepModelConfig.pm
# code for repeatmodeler 2 can be used when funannotate supports it.
#RUN wget http://www.repeatmasker.org/RepeatModeler/RepeatModeler-2.0.1.tar.gz && \
# tar xvfz RepeatModeler-2.0.1.tar.gz && \
# cd RepeatModeler-2.0.1 && \
# perl -i -0pe 's/^#\!.*/#\!\/usr\/bin\/env perl/g' \
# configure \
# BuildDatabase \
# Refiner \
# RepeatClassifier \
# RepeatModeler \
# TRFMask \
# util/dfamConsensusTool.pl \
# util/renameIds.pl \
# util/viewMSA.pl && \
# sed -i -e 's#/usr/local/RECON#/software/RECON-1.08/bin#g' RepModelConfig.pm && \
# sed -i -e 's#/usr/local/RepeatScout-1.0.6#/software/RepeatScout-1.0.6#g' RepModelConfig.pm && \
# sed -i -e 's#/usr/local/rmblast/bin#/software/rmblast-2.9.0-p2/bin#g' RepModelConfig.pm && \
# sed -i -e 's#/usr/local/RepeatMasker#/software/RepeatMasker#g' RepModelConfig.pm && \
# sed -i -e 's#/usr/local/bin/trf409.linux64#/usr/local/bin/trf#g' RepModelConfig.pm
# #cp RepModelConfig.pm.tmpl RepModelConfig.pm
#tRNA-Scan
RUN wget http://trna.ucsc.edu/software/trnascan-se-2.0.5.tar.gz && \
tar xvfz trnascan-se-2.0.5.tar.gz && \
cd tRNAscan-SE-2.0 && \
./configure && \
make && \
make install
#RUN git clone https://github.com/Gaius-Augustus/Augustus.git
#&& cd Augustus && git checkout v3.4.0 && \
# make augustus
# install missing perl modules:
# to be able to run genemarK:
#RUN cpanm YAML
# creating locales to prevent perl warning in singularity:
RUN apt-get install -y locales locales-all
ENV LC_ALL=en_US.UTF-8
ENV LANG=en_US.UTF-8
ENV LANGUAGE=en_US.UTF-8
#RUN rm /opt/conda/lib/python3.7/site-packages/funannotate/library.py
#ADD library.py /opt/conda/lib/python3.7/site-packages/funannotate/library.py
#possible workaround for conda augustus problem "compilation error"
RUN rm /opt/conda/bin/augustus && apt install -y augustus && ln -s /usr/bin/augustus /opt/conda/bin/augustus
# set paths specific to funannotate installation
ENV EVM_HOME="/opt/conda/opt/evidencemodeler-1.1.1"
ENV TRINITYHOME="/opt/conda/opt/trinity-2.8.5"
ENV QUARRY_PATH="/opt/conda/opt/codingquarry-2.0/QuarryFiles"
ENV ZOE="/opt/conda/bin/snap"
ENV PASAHOME="/opt/conda/opt/pasa-2.5.2"
ENV AUGUSTUS_CONFIG_PATH="/opt/conda/config"
ENV PERL5LIB="/opt/conda/lib/site_perl/5.26.2"
# set path specific to repeatmasker and repeatmodeler
ENV RMBLAST_DIR=/software/rmblast-2.9.0-p2/bin
ENV RECON_DIR=/software/RECON-1.08/bin
ENV PATH="/software/RepeatMasker:$PATH"
ENV PATH="/software/RepeatModeler-open-1.0.11:$PATH"
ENV REPEATMASKER_DIR="/software/RepeatMasker"
# set workdir and set paths to external dependencies which can be used with bindpoints
WORKDIR /data
ENV PATH="/data/external/gm_et_linux_64:$PATH"
ENV GENEMARK_PATH="/data/external/gm_et_linux_64"
ENV PATH="/data/external/signalp-4.1:$PATH"
ENV FUNANNOTATE_DB="/data/database"
ENV HOME="/data"
# fix missing perl module:
RUN cpanm local::lib
# install pigz
RUN mamba install pigz
# this fixes a problem with fasta2agp during the annotate step. There was a recent commit which should also fix this (https://github.com/nextgenusfs/funannotate/commit/ab0488b278d96d89ec6006199f0c22181aeca793)
#RUN sed -i 's#ACGTN#ACGTNRYSWKMBDHV#g' /opt/conda/lib/python3.7/site-packages/funannotate/aux_scripts/fasta2agp.pl