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Hi, we want to use tandem-genotypes to detect tandem repeats in genome-wide data. Following your instructions, I must first align my sequence using last before running tandem-genotypes. Unfortunately, last takes too long to align my sequence (fastq
files, 72G in size) within the server's maximum time limit (120 hours). In order to run tandem-Genotypes smoothly, I was wondering if you have any recommended alternatives for sequences alignment instead of last. Looking forward to your reply. Thanks a lot!
The text was updated successfully, but these errors were encountered:
I don't have a recommended alternative, but LAST should work fine for you. Some of our recent run times with LAST:
~15 hours for 76Gb data and ~24 hours for 112Gb (both with -P16 option).
So it should be fast enough...
We always use the "with repeat-masking" recipe for this kind of data, have you tried that?
Yes, I have tried the "with repeat-masking" recipe and other parameters to make last run faster. But for some reason, last is still too slow to finish within 120 hours for 72Gb data.
I wonder if I use Minimap2 for alignment and then convert the resulting PAF file to MAF format, will it affect the results of tandem-genotypes? Thanks a lot!
Hi, we want to use
tandem-genotypes
to detect tandem repeats in genome-wide data. Following your instructions, I must first align my sequence using last before runningtandem-genotypes
. Unfortunately, last takes too long to align my sequence (fastqfiles, 72G in size) within the server's maximum time limit (120 hours). In order to run tandem-Genotypes smoothly, I was wondering if you have any recommended alternatives for sequences alignment instead of last. Looking forward to your reply. Thanks a lot!
The text was updated successfully, but these errors were encountered: