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Files from a Guava EasyCyte HT causing exceptions #345

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germo006 opened this issue Nov 3, 2022 · 0 comments
Open

Files from a Guava EasyCyte HT causing exceptions #345

germo006 opened this issue Nov 3, 2022 · 0 comments

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@germo006
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germo006 commented Nov 3, 2022

Thank you for your bug report!

Please fill out the following template.

PLATFORM (Mac, PC, Linux, other): PC

OPERATING SYSTEM (eg OSX 10.7, Windows 8.1): Win 10

SEVERITY (Critical? Major? Minor? Enhancement?): Unsure

DESCRIPTION:

  • I've been trying to import data from a Guava EasyCyte HT.
  • I can export only "analyzed groups"
    -- These can be FCS 3.0, FCS 2.0, or .csv
  • The FCS 3.0 standard, I assume, is the best here. Cytoflow does not read this properly, seeing only the first well in the file. There should be 24, as the Guava software exports a 64 Mb file containing all the data from the run. The program can plot and play with that data, but only the very first well in the dataset. (B03 in the actual 96-well plate)
  • The FCS 2.0 standard exports each well analyzed as a separate .fcs file, which is more in line with what the docs imply I need. However, if I try to "add tubes" with any of these files, there's an error and I have to restart the program. The result is the same whether I try to use 1 or 24 files.
  • .csv option makes nice files with a row for each event detected, but they can't interact with Cytoflow.

Don't forget to attach the log file to this bug report!
NPG_3Nov2020.log

If you are having trouble with a particular FCS file, please attach that file too.
even zipped, the FCS 3.0 file is too large to upload to GitHub

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