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We are using hifi-asm to assemble human genome sequenced on Pacbio Revio machine, the coverage of the sample is roughly 30X (may be lower though), we found that hifi-asm might miss one haplotype in some low coverage region, eg the sample have two haplotypes, but the assembler reports the region only in one set of contigs. Is there any parameter setting (which I may miss), or internal hard-coded setting I can change, so the assembler can output contigs as aggressively as possible in both sets of haplotypes?
Thanks.
George
The text was updated successfully, but these errors were encountered:
I am not sure what I should give to you. One thing is one haplotype may be sequenced a little more than the other according to pacbio haplotagged bam file. We think there are two haplotypes is because there are noticeable number of heterozygous calls from the region (deepvariant calls).
Or if there is any log information we can check that anything has been dropped?
Hi there,
We are using hifi-asm to assemble human genome sequenced on Pacbio Revio machine, the coverage of the sample is roughly 30X (may be lower though), we found that hifi-asm might miss one haplotype in some low coverage region, eg the sample have two haplotypes, but the assembler reports the region only in one set of contigs. Is there any parameter setting (which I may miss), or internal hard-coded setting I can change, so the assembler can output contigs as aggressively as possible in both sets of haplotypes?
Thanks.
George
The text was updated successfully, but these errors were encountered: