Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Changes spades default mode from rna to meta #966

Open
yesimon opened this issue Jun 14, 2019 · 1 comment
Open

Changes spades default mode from rna to meta #966

yesimon opened this issue Jun 14, 2019 · 1 comment
Assignees

Comments

@yesimon
Copy link
Contributor

yesimon commented Jun 14, 2019

The rnaSpades mode is heavily focused on mRNA transcriptome assembly and is very aggressive at splicing assemblies into short transcripts. The —meta mode would be more suitable for our DNA/RNA assembly even though it is optimized for DNA metagenomes.

One barrier is that meta spades only accepts a single paired end library and doesn’t accept unpaired reads for now. Although not too common, we should handle this by warning after Trimmomatic if a significant number of reads become orphaned.

@notestaff
Copy link
Contributor

A branch adding metaspades is https://github.com/broadinstitute/viral-ngs/tree/is-add-metaspades

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

2 participants