-
Notifications
You must be signed in to change notification settings - Fork 0
/
FT-IM-MS GUI.py
688 lines (505 loc) · 23.4 KB
/
FT-IM-MS GUI.py
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177
178
179
180
181
182
183
184
185
186
187
188
189
190
191
192
193
194
195
196
197
198
199
200
201
202
203
204
205
206
207
208
209
210
211
212
213
214
215
216
217
218
219
220
221
222
223
224
225
226
227
228
229
230
231
232
233
234
235
236
237
238
239
240
241
242
243
244
245
246
247
248
249
250
251
252
253
254
255
256
257
258
259
260
261
262
263
264
265
266
267
268
269
270
271
272
273
274
275
276
277
278
279
280
281
282
283
284
285
286
287
288
289
290
291
292
293
294
295
296
297
298
299
300
301
302
303
304
305
306
307
308
309
310
311
312
313
314
315
316
317
318
319
320
321
322
323
324
325
326
327
328
329
330
331
332
333
334
335
336
337
338
339
340
341
342
343
344
345
346
347
348
349
350
351
352
353
354
355
356
357
358
359
360
361
362
363
364
365
366
367
368
369
370
371
372
373
374
375
376
377
378
379
380
381
382
383
384
385
386
387
388
389
390
391
392
393
394
395
396
397
398
399
400
401
402
403
404
405
406
407
408
409
410
411
412
413
414
415
416
417
418
419
420
421
422
423
424
425
426
427
428
429
430
431
432
433
434
435
436
437
438
439
440
441
442
443
444
445
446
447
448
449
450
451
452
453
454
455
456
457
458
459
460
461
462
463
464
465
466
467
468
469
470
471
472
473
474
475
476
477
478
479
480
481
482
483
484
485
486
487
488
489
490
491
492
493
494
495
496
497
498
499
500
501
502
503
504
505
506
507
508
509
510
511
512
513
514
515
516
517
518
519
520
521
522
523
524
525
526
527
528
529
530
531
532
533
534
535
536
537
538
539
540
541
542
543
544
545
546
547
548
549
550
551
552
553
554
555
556
557
558
559
560
561
562
563
564
565
566
567
568
569
570
571
572
573
574
575
576
577
578
579
580
581
582
583
584
585
586
587
588
589
590
591
592
593
594
595
596
597
598
599
600
601
602
603
604
605
606
607
608
609
610
611
612
613
614
615
616
617
618
619
620
621
622
623
624
625
626
627
628
629
630
631
632
633
634
635
636
637
638
639
640
641
642
643
644
645
646
647
648
649
650
651
652
653
654
655
656
657
658
659
660
661
662
663
664
665
666
667
668
669
670
671
672
673
674
675
676
677
678
679
680
681
682
683
684
685
686
687
688
import panel as pn
import numpy as np
from pyteomics import mzml
from scipy.signal import find_peaks, peak_widths
from scipy.interpolate import UnivariateSpline
import itertools, os
import seaborn as sns
import pandas as pd
import plotly.graph_objects as go
from lmfit.models import GaussianModel
from FTGUI_funcs import getMS_v2, getXIC, loadOrbiMS_v2, getXIC_Orbi, \
regrid, scanOrbi, zero_padding, getAFT, multiFT_data, XIC_preview, moCal
from CCS_funcs import calcThermalVelocity, calcAlpha, calcTransverseVelCoeff,\
calcNumberDensity, ccsFromDriftTime, calcCorrectedCCS
pn.extension('tabulator')
#========================================================================
#default current working directory - relative to root
# DEF_CWD = '~/Documents/GUI Testing'
DEF_CWD = '~/Documents'
#default m/z ranges to extract XICs from
DEF_MZ_VALS = [[298.0, 302.0], [354.0, 357.0], [410.0, 413.0],
[466.0, 469.0], [578.0, 582.0], [690.0, 694.0]]
#slicing off large values in frequency domain post-FT
ATD_SLICE = 30
#color palettes for XIC and ATD plots - see seaborn color palettes
XIC_COLORS = 'magma'
ATD_COLORS = 'icefire_r'
#first value in frequency sweep, it is generally assumed in FT-IM
#experiments that the start frequency is 5 Hz, hence the default argument
#see 'aFT' mode and 'startFreq' argument in "multiFT_data" function
START_FREQ = 5
#todo go through and change enable/disable options
#todo clear all plots when appropriate
#todo add option to normalize the ATD
#todo add XIC subtraction for better apodization
#containers for all content
main_grid = pn.GridSpec(nrows=9,
ncols=16,
sizing_mode='stretch_both')
main_tabs = pn.Tabs(('Main figures', main_grid))
fname_input = pn.widgets.TextInput(name='Filename',
placeholder = 'Enter csv filename',
sizing_mode = 'stretch_width')
exp_df_button = pn.widgets.Button(name='Export Fits',
margin = (24,5,5,5))
exp_button_row = pn.Row(fname_input,
exp_df_button)
df_viewer = pn.widgets.Tabulator(sizing_mode='stretch_width',
layout = 'fit_columns')
def fitsDF_exporter(event):
curDF = df_viewer.value
if len(fname_input.value) == 0:
curDF.to_csv('placeholder_name.csv', sep=',')
else:
curDF.to_csv(fname_input.value, sep=',')
exp_df_button.on_click(fitsDF_exporter)
df_divider = pn.layout.Divider()
export_df_col = pn.Column(exp_button_row,
df_divider,
df_viewer,
sizing_mode = 'stretch_width')
ccs_button = pn.widgets.Button(name='Calculate CCS',
button_type = 'success',
margin = (24,5,5,5))
charge_state_vals = pn.Card(title='m/z charge states')
neutral_amu_input = pn.widgets.FloatInput(name='Drift Gas Mass (amu)',
value = 28.0,
step = 1,
start = 1,
end = 200,
width = 250)
charge_state_vals.append(neutral_amu_input)
ccs_col = pn.Column(ccs_button,
charge_state_vals)
fit_tab_row = pn.Row(export_df_col, ccs_col,
sizing_mode = 'stretch_both')
main_tabs.append(('Fits view', fit_tab_row))
#initializing plotly figures =============================================
mzFig = go.Figure()
atdFig = go.Figure()
xicFig = go.Figure()
mzFig.update_xaxes(title='m/z', showline=True, linecolor='black')
mzFig.update_yaxes(title='Intensity (a.u.)', showline=True, linecolor='black',
linewidth=1.1)
mzFig.update_layout(template = 'plotly_white',
autosize=False,
width= 650,
height = 350,
margin=dict(t=25,r=5,b=5,l=5))
atdFig.update_layout(template = 'plotly_white',
autosize=False,
legend = dict(x=0.80,y=0.95),
width=980,
height=420,
margin=dict(t=5,l=5,b=5,r=5))
xicFig.update_layout(template = 'plotly_white',
autosize = False,
width=980,
height=200,
margin=dict(t=5,r=5,b=5,l=5))
#creating containers for each plotly plot
mzPane = pn.pane.Plotly(mzFig, height=350)
xicPane = pn.pane.Plotly(xicFig)
atdPane = pn.pane.Plotly(atdFig, width=980, height=450)
#temporary tab to maintain gui shape, will be cleared at XIC extraction
xicTabs = pn.Tabs(('curxic',xicPane))
file_input = pn.widgets.FileSelector(DEF_CWD,
refresh_period=5000,
height = 325)
#value argument specifies the default value of the radio buttons
# ms_type_radio = pn.widgets.RadioBoxGroup(name='Instrument',
# options=['HiRes', 'Std'],
# value = 'Std',
# inline=True,
# margin = 15,
# width = 100)
ms_type_radio = pn.widgets.RadioBoxGroup(name='Instrument',
options=['.mzML'],
value = '.mzML',
inline=True,
margin = 15,
width = 100)
load_mz_button = pn.widgets.Button(name='Load selected file',
sizing_mode='stretch_width',
margin=10)
current_dataset = {}
current_mzSpectrum = {}
def load_file(event):
current_dataset.clear()
current_mzSpectrum.clear()
mzFig.data = []
curFile = file_input.value[0]
fname = os.path.basename(curFile)
# if ms_type_radio.value == 'Std':
# spectral_data = [s for s in mzml.read(curFile)]
# mz_x, mz_y = getMS_v2(spectral_data)
if ms_type_radio.value == '.mzML':
mz_x, mz_y, spectral_data = loadOrbiMS_v2(curFile)
current_dataset['DATASET'] = spectral_data
#this is referenced again in CCS calc function
current_mzSpectrum['mz_x'] = mz_x
current_mzSpectrum['mz_y'] = mz_y
curTrace = go.Scatter(x=mz_x, y=mz_y,
line=dict(color='crimson', width=1))
mzFig.update_layout(title=fname)
mzFig.update_xaxes(range=(mz_x.min(), mz_x.max()))
mzFig.add_trace(curTrace)
load_mz_button.on_click(load_file)
file_load_row = pn.Row(load_mz_button, ms_type_radio)
mzranges_input = pn.widgets.ArrayInput(
name='XICs for the following m/z ranges will be extracted:',
max_array_size = 20,
value = np.array(DEF_MZ_VALS),
sizing_mode='stretch_width')
add_xlims_button = pn.widgets.Button(name='Add current m/z range to list',
sizing_mode='stretch_width',
button_type='default')
extract_xic_button = pn.widgets.Button(name='Extract XICs',
sizing_mode='stretch_width',
button_type='primary',
disabled=False)
def add_mz_xlims(event):
cur_min_mz, cur_max_mz = mzFig.layout.xaxis.range
print(cur_min_mz, cur_max_mz)
mzranges_input.value = np.append(mzranges_input.value,
[[round(cur_min_mz,1),
round(cur_max_mz,1)]],
axis=0)
add_xlims_button.on_click(add_mz_xlims)
xic_color_gen = sns.color_palette(XIC_COLORS, 10).as_hex()
xic_colors = itertools.cycle(xic_color_gen)
xic_trace_dict = {}
monoPeak_dict = {}
def extract_XICs(event):
xicTabs.clear()
xic_trace_dict.clear()
monoPeak_dict.clear()
mzx = current_mzSpectrum['mz_x']
mzy = current_mzSpectrum['mz_y']
for curmz_pair in mzranges_input.value:
mz1 = curmz_pair[0]
mz2 = curmz_pair[1]
mzCenter = (mz1+mz2)/2
mzTol = mz2 - mzCenter
# if ms_type_radio.value == 'Std':
# xic_x, xic_y = getXIC(current_dataset['DATASET'],
# mzCenter, tol=mzTol)
if ms_type_radio.value == '.mzML':
xic_x, xic_y = getXIC_Orbi(current_dataset['DATASET'],
mzCenter, tol=mzTol)
xicKey = 'm/z {:.1f}-{:.1f}'.format(mz1,mz2)
xic_trace_dict[xicKey] = (xic_x, xic_y)
#used for CCS calculations
mzLo_ind, mzHi_ind = np.searchsorted(mzx, [mz1, mz2])
maxPeakInd = np.argmax(mzy[mzLo_ind:mzHi_ind])
monoPeak = mzx[mzLo_ind:mzHi_ind][maxPeakInd]
monoPeak_dict[xicKey] = round(monoPeak,1)
cur_xicFig = go.Figure()
cur_xicFig.update_layout(template='plotly_white',
autosize=False,
legend=dict(x=0.85, y=0.95),
width=980,
height=200,
margin=dict(t=5,r=5,b=5,l=5))
cur_xicFig.update_xaxes(title='Time (s)',
showline=True,
linecolor='black')
cur_xicFig.update_yaxes(showline=True, linecolor='black')
cur_line = go.Scatter(x=xic_x, y=xic_y, name = xicKey,
line=dict(color=next(xic_colors), width=1))
cur_xicFig.add_trace(cur_line)
cur_xic_pane = pn.pane.Plotly(cur_xicFig, width=980, height=200)
xicTabs.append((xicKey, cur_xic_pane))
extract_xic_button.on_click(extract_XICs)
mz_opts_row = pn.Row(add_xlims_button, extract_xic_button,
sizing_mode='stretch_width')
mz_opts_col = pn.Column(mzranges_input, mz_opts_row,
sizing_mode='stretch_width')
apdz_checkbox = pn.widgets.Checkbox(name='Apodization',
width=100,
margin= (22,5,5,50))
apdz_list = ['bartlett', 'blackman', 'hamming', 'hanning',
'barthann', 'bohman', 'nuttall', 'parzen', 'tukey']
apdz_type = pn.widgets.Select(name='Type:',
options=apdz_list,
value = 'hanning',
width = 150)
zpad_checkbox = pn.widgets.Checkbox(name='Zero padding',
width=100,
margin=(22,5,5,10))
zpad_len_box = pn.widgets.FloatInput(name='Pad length', value=1, step=1,
start=0, end=10,
width=100)
xic_change_button = pn.widgets.Button(name='Apply XIC changes / Confirm XIC',
button_type='primary',
margin=(22,5,5,5),
disabled=False)
xic_reset_button = pn.widgets.Button(name='Reset XICs',
margin=(22,5,5,5),
width=100,
disabled=True)
xic_mods_row = pn.Row(apdz_checkbox, apdz_type,
zpad_checkbox, zpad_len_box,
xic_change_button, xic_reset_button,
margin=(5,5,0,5))
xic_mod_div = pn.layout.Divider(margin=(5,5,0,22))
def xic_changer(event):
for curTab in xicTabs:
curDataContainer = curTab.object.data[0]
curDataKey = curDataContainer.name
x_orig, y_orig = xic_trace_dict[curDataKey]
y_mod = XIC_preview(amp = y_orig,
windowBool = apdz_checkbox.value,
window = apdz_type.value,
padBool = zpad_checkbox.value,
padLen = zpad_len_box.value)
if zpad_checkbox.value:
curDataContainer.x = None
curDataContainer.figure.update_xaxes(title='Bins')
curDataContainer.y = y_mod
xic_change_button.disabled = True
xic_reset_button.disabled = False
plot_ATD_button.disabled = False
xic_change_button.on_click(xic_changer)
def xic_resetter(event):
for curTab in xicTabs:
curDataContainer = curTab.object.data[0]
curDataKey = curDataContainer.name
x_orig, y_orig = xic_trace_dict[curDataKey]
curDataContainer.x = x_orig
curDataContainer.y = y_orig
curDataContainer.figure.update_xaxes(title='Time (s)')
xic_change_button.disabled = False
xic_reset_button.disabled = True
plot_ATD_button.disabled = True
xic_reset_button.on_click(xic_resetter)
sRate_input = pn.widgets.FloatInput(name='Sweep Rate (Hz/s)',
width= 150,
value=10,
step=0.5,
start=0.1,
end=100)
length_input = pn.widgets.FloatInput(name='Length (cm)',
width=120,
value=17.4,
step=0.1,
start=0.1,
end=100)
voltage_input = pn.widgets.FloatInput(name='Voltage (V)',
width=120,
value=1000,
step=1,
start=0.1,
end=50000)
temp_input = pn.widgets.FloatInput(name='Temperature (C)',
width=120,
value=25.0,
step=1,
start=-273.15,
end=1000)
press_input = pn.widgets.FloatInput(name='Pressure (Torr)',
width=120,
value = 700,
step = 1,
start=0,
end=10000)
tOffset_input = pn.widgets.FloatInput(name='tOffset',
width=120,
value=0.1,
step=0.1,
start=0,
end=10,
disabled=True)
expParams_row = pn.Row(sRate_input, length_input, voltage_input,
temp_input, press_input, tOffset_input,
margin=(0,0,0,50))
plot_ATD_button = pn.widgets.Button(name='Reconstruct ATD',
button_type='success',
min_width = 400,
disabled = True)
only_FT_checkbox = pn.widgets.Checkbox(name='FT only',
margin=(15,5),
width=70)
aFT_checkbox = pn.widgets.Checkbox(name='aFT',
width=60,
margin=(15,0),
disabled=True)
palette_gen = sns.color_palette(ATD_COLORS, 10).as_hex()
atd_colors = itertools.cycle(palette_gen)
#todo add functionality for aFT
atd_traces = {}
def ATD_plotter(event):
atd_traces.clear()
atdFig.data = []
if only_FT_checkbox.value:
atd_xlabel = 'Frequency (Hz)'
else:
atd_xlabel = 'Time (ms)'
sRate = sRate_input.value
atdFig.update_xaxes(title=atd_xlabel)
atdFig.update_yaxes(title='Intensity (a.u.)')
for key, val in xic_trace_dict.items():
time, amp = val
ft_x, ft_y = multiFT_data(time = time, amp = amp,
sweepRate=sRate,
method = 'FFT',
windowBool=apdz_checkbox.value,
window = apdz_type.value,
padBool = zpad_checkbox.value,
padLen = zpad_len_box.value,
startFreq = START_FREQ,
tOffset = tOffset_input.value)
if only_FT_checkbox.value:
FT_x = ft_x[ATD_SLICE:] #keeping as frequency domain
else:
FT_x = (ft_x[ATD_SLICE:]/sRate)*1000 #converting to time (ms)
FT_y = ft_y[ATD_SLICE:]
atd_traces[key] = (FT_x, FT_y)
atdLine = go.Scatter(x=FT_x, y=FT_y, name=key,
line=dict(color=next(atd_colors), width=1))
atdFig.add_trace(atdLine)
plot_ATD_button.on_click(ATD_plotter)
min_dist_input = pn.widgets.FloatInput(name='Min distance',
value = 1,
width = 100)
min_width_input = pn.widgets.FloatInput(name='Min width',
value = 1,
width = 100)
min_prom_input = pn.widgets.FloatInput(name='Prominence',
value = 0.4,
step = 0.1,
end = 1.0,
width = 100)
update_fits_button = pn.widgets.Button(name='Update fits',
width = 100)
fits_dropdown = pn.Card(min_dist_input,
min_width_input,
min_prom_input,
update_fits_button,
collapsed = True,
title = 'Fit Params',
max_width = 200,
sizing_mode = 'stretch_width')
fit_peaks_button = pn.widgets.Button(name='Fit Peaks',
width=100,
margin=(5,5))
atd_reset_button = pn.widgets.Button(name='Reset plot',
width=100,
margin=(5,5))
def atd_resetter(event):
atdFig.data = atdFig.data[:len(atd_traces.keys())]
atd_reset_button.on_click(atd_resetter)
def peak_fitter(event):
resultsList = []
#removing any peak fitting traces, keys should only reflect data
atdFig.data = atdFig.data[:len(atd_traces.keys())]
# charge_state_vals.clear()
#required to keep drift gas amu input box initialized at start of script
charge_state_vals.objects = [charge_state_vals.objects[0]]
for k, (key, val) in enumerate(atd_traces.items()):
xvec, yvec = val
scaleFactor = yvec.max()
y_norm = yvec/scaleFactor
peakInds,_ = find_peaks(y_norm,
prominence = min_prom_input.value,
width = min_width_input.value,
distance = min_dist_input.value)
width_results = peak_widths(y_norm, peakInds, rel_height=0.5)[0]
#average delta time to calculate peak width
avg_del_xvec = np.diff(xvec).mean()
#convert to std dev for fitting instead of fwhm
raw_sigma_est = (width_results*avg_del_xvec)/2.35482
for cp, curParams in enumerate(zip(peakInds, raw_sigma_est)):
curAnalyte = 'Peak{}_'.format(cp+1) #arb name
curGaussMod = GaussianModel(prefix=curAnalyte)
if cp == 0:
# initializing the composite fit model
pars = curGaussMod.guess(y_norm, x=xvec)
else:
pars.update(curGaussMod.make_params())
raw_dt = xvec[curParams[0]]
raw_sigma = curParams[1]
raw_amp = y_norm[curParams[0]]
pars['{}center'.format(curAnalyte)].set(value=raw_dt,
min=raw_dt - 0.1,
max=raw_dt + 0.1)
pars['{}sigma'.format(curAnalyte)].set(value=raw_sigma)
pars['{}amplitude'.format(curAnalyte)].set(value=raw_amp)
if cp == 0:
mod = curGaussMod
else:
mod += curGaussMod
init = mod.eval(pars, x=xvec)
out = mod.fit(y_norm, pars, x=xvec)
comps = out.eval_components(x=xvec)
fitLine = go.Scatter(x=xvec, y=comps[curAnalyte]*scaleFactor,
name = key+' - fit',
line=dict(color='crimson', width=1))
atdFig.add_trace(fitLine)
ctr = out.params['{}center'.format(curAnalyte)].value
fwhm = out.params['{}fwhm'.format(curAnalyte)].value
K0 = moCal(dtime=ctr, length = length_input.value,
voltage = voltage_input.value,
T = temp_input.value + 273.15, P = press_input.value)
temp_dict = {'m/z Range' : key,
'Peak': curAnalyte,
'Drift Time': ctr,
'K0': K0,
'RP': ctr/fwhm,
'Monoisotopic Peak': monoPeak_dict[key]}
resultsList.append(temp_dict)
curFloatInput = pn.widgets.IntInput(name = key,
value = 1,
step = 1,
start = 1,
end = 200,
width = 250)
charge_state_vals.append(curFloatInput)
columns = ['Monoisotopic Peak', 'm/z Range', 'Peak',
'Drift Time', 'K0', 'RP']
df = pd.DataFrame(resultsList, columns=columns)
DF = df.sort_values(by=['m/z Range', 'Peak'], ignore_index=True)
df_viewer.value = DF
fit_peaks_button.on_click(peak_fitter)
update_fits_button.on_click(peak_fitter)
vdvtfc = np.loadtxt('velocityRatio.csv', skiprows=1, delimiter=',')
vdvtCalc = vdvtfc[:, 0]
fcCalc = vdvtfc[:, 1]
f = UnivariateSpline(vdvtCalc, fcCalc, s=0)
xnew = np.linspace(vdvtCalc.min(), vdvtCalc.max(),
len(vdvtCalc) * 100) #increase the length of vdvtCalc
fcCalcNew = f(xnew)
def ccs_calc(event):
curDF = df_viewer.value
zDict = {}
for zBox in charge_state_vals.objects:
zDict[zBox.name] = zBox.value
curDF['Charge State'] = [zDict[pk] for pk in curDF['m/z Range']]
curDF['CCS A^2'] = calcCorrectedCCS(
ionMass=curDF['Monoisotopic Peak'] * curDF['Charge State'],
ionCharge=curDF['Charge State'],
gasMass=neutral_amu_input.value,
driftLength=length_input.value,
driftPotential=voltage_input.value,
driftTime=curDF['Drift Time']/1000,
gasPress=press_input.value,
gasTemp=temp_input.value + 273.15,
fcList=[xnew, fcCalcNew],
debug=False)
df_viewer.value = curDF
ccs_button.on_click(ccs_calc)
plot_atd_row = pn.Row(plot_ATD_button,
only_FT_checkbox,
aFT_checkbox,
fit_peaks_button,
atd_reset_button,
fits_dropdown)
ft_opts_col = pn.Column(xic_mods_row, xic_mod_div, expParams_row, plot_atd_row)
left_col = pn.Column(file_input, file_load_row, mzPane, mz_opts_col)
right_col = pn.Column(xicTabs, ft_opts_col, atdPane)
main_grid[0:8, 0:6] = left_col
main_grid[0:8, 6:15] = right_col
# app = pn.panel(main_tabs)
# server = app.show(threaded=True)
pn.serve(main_tabs)