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view qseq uses letters ARND instead of ACTG #798

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imLew opened this issue Mar 22, 2024 · 2 comments
Open

view qseq uses letters ARND instead of ACTG #798

imLew opened this issue Mar 22, 2024 · 2 comments

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@imLew
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imLew commented Mar 22, 2024

User diamond version 2.1.7 diamond view --daa ... --outfmt qseq produces sequences that contain the letters ARND instead of ACTG.

When I run diamond --outfmt qseq on the fastq files used to produce the daa file this does not happen. Furthermore the sequences have the same lengths and is a 1 to 1 correspondence between the letters (save a few Ns in the sequences using ACTG), so I don't think they are ambiguity codes.

@imLew
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imLew commented Apr 4, 2024

example.zip
Here is an example running diamond view --outfmt 6 qseq -a this should produce sequences with ARND.

@terrycojones
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@bbuchfink Hi Benjamin. Do you have any idea about this issue? I hope you're well! Terry

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