isopretGUI reports slightly different numbers for the same input on different machines

[hansenp]

We noticed that isopretGUI reports slightly different numbers of differentially expressed genes, differentially spliced genes, etc. for the same input file from HBA-Delas on different machines (table at the top of the analysis screen).

For file SRP119676_240.txt from the Zenodo repository with MD5 checksum 987bbc84fc2595a137f7eae2ae314013, the following numbers were reported on a Mac M2:

And on a Core i7 machine, the following numbers were reported:

In both cases the dmg file for isopretGUI was created using bash package.sh.

To narrow down the error further, it would be good if others could create such screenshots using the same file and post them here.

[pnrobinson]

This is worrisome, I will try to track this down.
I am thinking it might be better to try and improve the modularity of the code for better testing.

[hansenp]

So far we have only tested on two machines and got different results. You could try to reproduce the error from above on your machine. Maybe that will give us a clue.

[pnrobinson]

@hansenp I tested the above examples on my M1 mac. Note sure if this is a mistake but you are showing different genes (CS and UQCRQ on top but GK and FGGY on the bottom). In any case I get the same numbers as shown above.

[hansenp]

I tested again with the same file (SRP119676_240.txt) on the same computer (M2) as above, but with the latest version of isopretGUI. Here is the resulting table with the summary statistics:

Only the values for Significant DGE GO Terms and Significant DAS GO Terms changed slightly. The remaining values are unchanged.

@pnrobinson Do you get the same results on your computer?