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Support for FCS 3.2? #156

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alefrol638 opened this issue Apr 25, 2024 · 4 comments
Open

Support for FCS 3.2? #156

alefrol638 opened this issue Apr 25, 2024 · 4 comments

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@alefrol638
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Dear CytoML Team,

I am trying to import gates from a workspace, which works with FCS files from the new BD software FACS CHORUS. The FCS files are FCS 3.2 and the function gives me the error, that the format is wrong:

 gs  <- flowjo_to_gatingset(open_flowjo_xml(wsp, sample_names_from="sampleNode"), name = 1,mc.cores = 7,path = <pathToFile>) #flowWorkspace package

Error: This does not seem to be a valid FCS2.0, FCS3.0 or FCS3.1 file

Is there a way to work with this workspace/FCS files?

Thanks!

@mikejiang
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We don't have official support for 3.2 yet, did relax the check in flowcore RGLab/flowCore#263 so that it can be load there, I can do a similar change in cytolib to allow your workspace parsing proceed, but no guarantee it will work if your fcs is not fully compatible with 3.1

@alefrol638
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all right, I would give it a try

@mikejiang
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RGLab/cytolib#58

@mikejiang
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I pushed the cytolib change to bioconductor develop branch:
To trigger the rebuild for CytoML, I bumped its version to 2.15.3 as well

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