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Dear team,
I am working on some data of mine using my own homebrew adapters. I have noticed that fastp has no leniency in errors (PCR or oligo synthesis?) in the adapter recognition and removal. When analysing data in fastQC I see over-representation of my adapter sequence with minor mutations (indels). See the example below.
Is there any way you could add a mismatch value? Like allowing for 1 or 2 mutations?
Dear team,
I am working on some data of mine using my own homebrew adapters. I have noticed that fastp has no leniency in errors (PCR or oligo synthesis?) in the adapter recognition and removal. When analysing data in fastQC I see over-representation of my adapter sequence with minor mutations (indels). See the example below.
Is there any way you could add a mismatch value? Like allowing for 1 or 2 mutations?
Example:
GATCGGAAGAGCACACGTCTGAACTCCAGTCAC
GATCGGAAGAGCACACGTCT AACTCCAGTCAC
GATCGGAAGAGCACACGTC GAACTCCAGTCAC
GATCGGAAGAGCACACGTCTGAACCCAGTCAC
GATCGGAAGAGCACAC TCTGAACTCCAGTCAC
GATCGGAAGAGCACACGTCT AACTCCAGTCA
GATCGGAAGAGCACACGTCTGAACTC AGTCAC
GATCGGAAGAGCACACG CTGAACTCCAGTCAC
GATCGGAAGAGCACACGTCTGA CTCCAGTCAC
GATCGGAAGAGCACACGT TGAACTCCAGTCAC
GATCGGAAGAGCACA GTCTGAACTCCAGTCAC
Thank you!!
Cheers
Kor
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