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Variable Mods for glycopeptides #1248
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Open search should return your glycans. Did you make sure that the mass window was large enough to detect glycopeptides? If you're modifying the glycan chains to essentially create new glycan monomers, I think you're going to introduce additional issues with glycan assignment as well. |
The mass window shouldn't be an issue since I am able to detect glycopeptides with other protocols using the same mass window. The issue I am having sounds more like your second point. |
Hi, Best, |
That new update sounds fantastic, I'll be sure to check it out upon release. Looking at the delta masses sounds like a good idea, thanks for the support! -Alex |
Support for user-defined glycan modifications (fixed or variable) is now available in the 22.0 release, and I will post some documentation explaining how to use the new features to the glyco tutorial later today. Best, |
Hi-
I've been using the fragpipe N-Glyco-HCD/LFQ and O-Glyco-HCD workflows for glycopeptide analysis. Some of my protocols involve chemical modifications to terminal glycans. I am wondering if there is a way to add a variable modification to a glycan in these workflows. I believe the only way seems to be manually changing the mass offsets of the glycans to represent the chemically modified glycan. I've tried open search too, but that does not yield any results.
Is there any way to create a variable mod for glycans? If not, is there any other method supported aside from adding all possible mass offsets?
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