You signed in with another tab or window. Reload to refresh your session.You signed out in another tab or window. Reload to refresh your session.You switched accounts on another tab or window. Reload to refresh your session.Dismiss alert
Hi all
I was digging in the write.hdf5 function to optimize my merged channel signal. Since many of these channel intensities are skewed beyond repair, I gave a z-score normalization appoach a try, and it turned out quite ok (we dont have memebrane markers in the panel, so I try my best to find a good marker combination).
I am doing similar things like the default function with the min-max normalized channels: clipping the z-scores channel-wise (within the rw.tbl loop), as well as the merged signal intensities in rw.sms.
This approach works nicely in my ROIs, but the thing I struggle is the following: In order to check parameter combinations, I have to export the hdf5 crops and load them into ilastik.... I would like to have an option where the write.hdf5 with plot=T
takes the AR array, actually I just need the Merged channel in there, and plot this channel.
That would allow to run a bunch of different paramters against each other and then just have a look at the merged channel. How do I access the data in AR so that I can plot the merged channel?
Sorry for such a detailed request...
Best matthias
The text was updated successfully, but these errors were encountered:
@mabar1 there is actually an argument in the write.hdf5 function that will allow you to create a merged channel, using whichever combination of channels you like.
Hi all
I was digging in the write.hdf5 function to optimize my merged channel signal. Since many of these channel intensities are skewed beyond repair, I gave a z-score normalization appoach a try, and it turned out quite ok (we dont have memebrane markers in the panel, so I try my best to find a good marker combination).
I am doing similar things like the default function with the min-max normalized channels: clipping the z-scores channel-wise (within the
rw.tbl
loop), as well as the merged signal intensities inrw.sms
.This approach works nicely in my ROIs, but the thing I struggle is the following: In order to check parameter combinations, I have to export the hdf5 crops and load them into ilastik.... I would like to have an option where the
write.hdf5
withplot=T
takes the
AR
array, actually I just need the Merged channel in there, and plot this channel.That would allow to run a bunch of different paramters against each other and then just have a look at the merged channel. How do I access the data in
AR
so that I can plot the merged channel?Sorry for such a detailed request...
Best matthias
The text was updated successfully, but these errors were encountered: