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10x v2 filter part in config.yaml #100

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YOU-k opened this issue Jan 20, 2020 · 2 comments
Open

10x v2 filter part in config.yaml #100

YOU-k opened this issue Jan 20, 2020 · 2 comments

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@YOU-k
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YOU-k commented Jan 20, 2020

Hi,
I would like to run dropseqpipe for 10x v2 data. and i am not sure about what to be included in config.yaml. Here is what I have,
FILTER: barcode-whitelist: '' 5-prime-smart-adapter: '' cell-barcode: start: 1 end: 16 UMI-barcode: start: 17 end: 26 cutadapt: adapters-file: R1: quality-filter: 30 maximum-Ns: 1 extra-params: '' R2: quality-filter: 30 minimum-adapters-overlap: 6 minimum-length: 15 extra-params: ''
but I would get
SyntaxError: Input and output files have to be specified as strings or lists of strings.
i am not sure if empty cutadapt file is the reason for this.
is there any suggestion for me?
Thanks!

@seb-mueller
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An empty adapter file can well be the reason for it. Could you try with a non empty one? There are some in the template directory such as this one to try. Having said this, we should probably implement proper checks with meaningful error messages for this kind of thing. Please let us know if this was indeed the reason if you find out.

@YOU-k
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YOU-k commented Feb 24, 2020

a randomly not empty one would work. but i have to check the stats for the percentage of trimmed sequences in case if some wanted sequences are trimmed accidentally.

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