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Augustus in Braker encountered problems #360

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17863952296 opened this issue Sep 4, 2022 · 0 comments
Open

Augustus in Braker encountered problems #360

17863952296 opened this issue Sep 4, 2022 · 0 comments

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@17863952296
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when I the run the braker, the problem was encountered.
The input: braker.pl --cores=16 --species=Cyclidium --genome=Cy_Canu.filter.fasta --bam=all.bam Cy_hisat2.sort.bam
The output: # WARNING: Number of reliable training genes is low (560). Recommended are at least 600 genes
#*********
ERROR in file /usr/local/braker/scripts/braker.pl at line 7729
Failed to execute: /usr/bin/python3 /usr/local/augustus/scripts/fix_in_frame_stop_codon_genes.py -g /apps/users/class00/dzg/Cyclidium/RNAseq/Braker2/braker/genome.fa -t /apps/users/class00/dzg/Cyclidium/RNAseq/Braker2/braker/augustus.hints.gtf -b /apps/users/class00/dzg/Cyclidium/RNAseq/Braker2/braker/bad_genes.lst -o augustus.hints_fix_ifs_ -s Cyclidium_sp -m off --UTR off --print_utr off -a /usr/local/augustus/config -C /usr/local/cdbfasta -A /usr/local/augustus/config/../bin -S /usr/local/augustus/config/../scripts -H /apps/users/class00/dzg/Cyclidium/RNAseq/Braker2/braker/hintsfile.gff -e /usr/local/braker/scripts/cfg/rnaseq.cfg > /apps/users/class00/dzg/Cyclidium/RNAseq/Braker2/braker/fix_in_frame_stop_codon_genes_augustus.hints.log 2> /apps/users/class00/dzg/Cyclidium/RNAseq/Braker2/braker/errors/fix_in_frame_stop_codon_genes_augustus.hints.err

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