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Issue with CTF estimation, RELION tries to convert particles even though they are in the right format #1083

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nmorano11 opened this issue Feb 15, 2024 · 1 comment

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@nmorano11
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This is a template for reporting bugs. Please fill in as much information as you can.

Hello, I recently started processing some data. However, everytime I try to run CTF estimation, I get an error where Relion thinks that the frames are from a previous version of Relion. It then tries to convert them and then errors out and says "Star file is already at version 3.0001.

Please write a clear description of what the problem is.
Data processing questions should be posted to the CCPEM mailing list, not here.
DO NOT cross post a same question to multiple issues and/or many mailing lists (CCPEM, 3DEM, etc).

Environment:

  • OS: [dont know]
  • MPI runtime: [e.g. OpenMPI 2.0.1]
  • RELION version [RELION-3.1]
  • Memory: [1000 TB]
  • GPU: [dont know]

Dataset:

  • Pixel size: [e.g. 0.83 Å/px]
  • Description: [e.g. A dimeric protein of about 200 kDa in total]

WARNING: MotionCorr/job007/NORO_121823/23dec12b_noro3_4_00003gr_00064sq_v02_00002hln_v01_00002enn_frames.star seems to be from a previous version of Relion. Attempting conversion...
You should make sure metadata in the optics group table after conversion is correct.
in: /relion/src/jaz/io/star_converter.cpp, line 14
ERROR:
StarConverter::convert_3p0_particlesTo_3p1: Star file is already at version 3.0001
=== Backtrace ===
/relion/build/bin/relion_run_ctffind(_ZN11RelionErrorC1ERKNSt7__cxx1112basic_stringIcSt11char_traitsIcESaIcEEES7_l+0x66) [0x491ce6]
/relion/build/bin/relion_run_ctffind(_ZN13StarConverter27convert_3p0_particlesTo_3p1ERK13MetaDataTableRS0_S3_NSt7__cxx1112basic_stringIcSt11char_traitsIcESaIcEEEb+0x29b) [0x4ed72b]
/relion/build/bin/relion_run_ctffind(_ZN16ObservationModel10loadSafelyENSt7__cxx1112basic_stringIcSt11char_traitsIcESaIcEEERS_R13MetaDataTableS5_ib+0x325) [0x4d4435]
/relion/build/bin/relion_run_ctffind(_ZN13CtffindRunner10initialiseEv+0x237) [0x484f37]
/relion/build/bin/relion_run_ctffind(main+0x33) [0x470bf3]
/lib64/libc.so.6(__libc_start_main+0xf3) [0x7f5051ea26a3]
/relion/build/bin/relion_run_ctffind(_start+0x2e) [0x47390e]

ERROR:
StarConverter::convert_3p0_particlesTo_3p1: Star file is already at version 3.0001

==================

@biochem-fan
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Please respect our reporting template.

OS: [dont know]
MPI runtime: [e.g. OpenMPI 2.0.1]
RELION version [RELION-3.1]
Memory: [1000 TB]
GPU: [dont know]

This is not useful at all. We need details to investigate.
Also note that RELION 3.1 is no longer supported.

I guess you specified a wrong STAR file to the CtfFind job.
Did you really specify corrected_micrographs.star from the MotionCorr job?

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